Tyrosyl phosphorylation and activation of a type II phosphatidylinositol 4-kinase by p56(lck) in concanavalin A stimulated rat splenic lymphocytes
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Title |
Tyrosyl phosphorylation and activation of a type II phosphatidylinositol 4-kinase by p56(lck) in concanavalin A stimulated rat splenic lymphocytes
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Creator |
FERNANDIS, AZ
SUBRAHMANYAM, G |
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Subject |
cell antigen-receptor
signal-transduction protein-kinase purification binding spleen t-cell activation phosphorylation signal transduction |
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Description |
Recent evidence suggests that concanavalin A modulates tyrosyl phosphorylation and activation of a type II PtdIns 4-kinase in rat splenic lymphocytes. However, the regulatory protein tyrosine kinase(s) remain to be elusive. The present manuscript provides evidence that a type II PtdIns 4-kinase associates with p56(lck) in Con A stimulated rat splenic lymphocytes. In vitro phosphorylation studies suggest that p561(lck) regulates phosphorylation and activation of type II PtdIns 4-kinase. Inhibition of p561(lck) activity in vivo has shown to reduce tyrosyl phosphorylation and activation of PtdIns 4-kinase by Con A. These results suggest that p56(lck) may be the physiological regulator of type II PtdIns 4-kinase. (. .
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Publisher |
PERGAMON-ELSEVIER SCIENCE LTD
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Date |
2011-08-27T02:17:20Z
2011-12-26T12:57:41Z 2011-12-27T05:44:43Z 2011-08-27T02:17:20Z 2011-12-26T12:57:41Z 2011-12-27T05:44:43Z 2000 |
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Type |
Article
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Identifier |
MOLECULAR IMMUNOLOGY, 37(6), 273-280
0161-5890 http://dx.doi.org/10.1016/S0161-5890(00)00053-5 http://dspace.library.iitb.ac.in/xmlui/handle/10054/11509 http://hdl.handle.net/10054/11509 |
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Language |
en
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