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Reactive oxygen species induced by shear stress mediate cell death in Bacillus subtilis

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Title Reactive oxygen species induced by shear stress mediate cell death in Bacillus subtilis
 
Creator SAHOO, SUSMITA
RAO, KRISHNAMURTHY K
SURAISHKUMAR, GK
 
Subject bacteria
cell culture
cytology
fragrances
shear stress
proteins
 
Description Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of a caspase-3-like protease (C3LP) followed by DNA fragmentation. Shear stress leads to an increase in specific intracellular reactive oxygen species (siROS), possibly through activation of NADH oxidase (NOX). The formation of siROS precedes the activation of C3LP and DNA fragmentation, thus establishing siROS as the molecular link between shear stress and apoptosis-like cell death. A model is proposed in which NOX is viewed as being strategically placed on the plasma membrane of B. subtilis that senses and converts a mechanical force arising from shear stress into a chemical signal leading to activation of C3LP, DNA fragmentation, and thus, apoptosis-like cell death.
 
Publisher Wiley
 
Date 2009-11-26T11:23:15Z
2011-11-25T15:56:44Z
2011-12-26T13:01:29Z
2011-12-27T05:44:59Z
2009-11-26T11:23:15Z
2011-11-25T15:56:44Z
2011-12-26T13:01:29Z
2011-12-27T05:44:59Z
2006
 
Type Article
 
Identifier Biotechnology and Bioengineering 94(1), 118-127
1097-0290
10.1002/bit.20835
http://hdl.handle.net/10054/1727
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1727
 
Language en