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Fold-recognition and comparative modeling of human β3GalT I, II, IV, V and VI and β3GalNAcT I: prediction of residues conferring acceptor substrate specificity

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Title Fold-recognition and comparative modeling of human β3GalT I, II, IV, V and VI and β3GalNAcT I: prediction of residues conferring acceptor substrate specificity
 
Creator PATEL, RONAK Y
BALAJI, PETETY V
 
Subject mapping
mutagenesis
data acquisition
mathematical models
 
Description β3GalTs are type II transmembrane proteins that transfer galactose from UDP-Gal donor substrate to acceptor GlcNAc, GalNAc or Gal in β1 → 3-linkage. β1 → 3-linked galactose have been found to be a part of many glycans like glycosphingolipids, core tetrasaccharide of proteoglycans, type 1 chains. The 3-D structure of none of the β3GalTs is known to date. In this study, the 3-D structures of human β3GalT I, II, IV, V, VI and β3GalNAcT I have been modeled using fold-recognition and comparative modeling methods. Residues that constitute the UDP-Gal binding site have been predicted. The models are able to qualitatively rationalize data from the site-directed mutagenesis experiments reported in the literature. Residues likely to be involved in conferring differential acceptor substrate specificity have been predicted by a combination of specificity determining positions prediction (SDPs) and subsequent mapping on the generated 3-D models.
 
Publisher Elsevier
 
Date 2009-04-23T11:40:22Z
2011-12-08T06:25:20Z
2011-12-26T13:01:31Z
2011-12-27T05:45:02Z
2009-04-23T11:40:22Z
2011-12-08T06:25:20Z
2011-12-26T13:01:31Z
2011-12-27T05:45:02Z
2007
 
Type Article
 
Identifier Journal of Molecular Graphics and Modelling 26(1), 255-268
1093-3263
10.1016/j.jmgm.2006.12.003
http://hdl.handle.net/10054/1212
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1212
 
Language en