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Silanization and antibody immobilization on SU-8

DSpace at IIT Bombay

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Field Value
 
Title Silanization and antibody immobilization on SU-8
 
Creator JOSHI, M
PINTO, RICHARD
RAMGOPAL RAO, V
MUKHERJI, SOUMYA
 
Subject antibodies
hydrolysis
silanes
sensors
microelectromechanical devices
 
Description SU-8, an epoxy based negative photoresist, has emerged as a structural material for microfabricated sensors due to its attractive mechanical properties like low Young's modulus and chemical properties like inertness to various chemicals used in microfabrication. It can be used to fabricate MEMS structures of high aspect ratio. However, the use of SU-8 in BioMEMS application has been limited by the fact that immobilization of biomolecules on SU-8 surfaces has not been reported. In this study, the epoxy groups on the SU-8 surface were hydrolyzed in the presence of sulphochromic solution. Following this, the surface was treated with [3-(2-aminoethyl) aminopropyl]-trimethoxysilane (AEAPS). The silanized SU-8 surface was used to incubate human immunoglobulin (HIgG). The immobilization of HIgG was proved by allowing FITC tagged goat anti-human IgG to react with HIgG. This process of antibody immobilization was used to immobilize HIgG on microfabricated SU-8 cantilevers.
 
Publisher Elsevier
 
Date 2009-04-23T11:41:01Z
2011-12-08T06:25:21Z
2011-12-26T13:01:32Z
2011-12-27T05:45:06Z
2009-04-23T11:41:01Z
2011-12-08T06:25:21Z
2011-12-26T13:01:32Z
2011-12-27T05:45:06Z
2007
 
Type Article
 
Identifier Applied Surface Science 253(6), 3127-3132
0169-4332
10.1016/j.apsusc.2006.07.017
http://hdl.handle.net/10054/1216
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1216
 
Language en