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Volumetric properties of amino acids and hen-egg white lysozyme in aqueous Triton X-100 at 298.15 K

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Title Volumetric properties of amino acids and hen-egg white lysozyme in aqueous Triton X-100 at 298.15 K
 
Creator SINGH, SK
KISHORE, N
 
Subject partial molar volumes
bovine serum-albumin
sodium dodecyl-sulfate
thermodynamic properties
heat-capacities
surfactants
peptides
proteins
25-degrees-c
pressure
standard partial molar volume
alpha-amino acids
partial specific volume
hydration
triton x-100
lysozyme
 
Description The apparent molar volumes, V-phi,V-2, of glycine, alanine, alpha-amino-n-butyric acid, valine, leucine, and lysine monohydrochloride have been determined in aqueous solutions of 0.05, 0.1, and 0.4 mol-kg(-1) Triton X-100 (TX-100), and the partial specific volume, v(0), of hen-egg-white lysozyme in 0.4 mol- kg(-1) TX-100 by density measurements at 298.15 K. These data have been used to calculate the infinite dilution apparent molar volumes, V-2,m(0), for the amino acids in aqueous TX-100 solutions and the standard partial molar volumes of transfer, Delta(tr)V(2,m)(0), of the amino acids from water to the aqueous surfactant solutions. The linear correlation of V-2,m(0) for a homologous series of amino acids has been utilized to calculate the contribution of the charged end groups (NH3+, COO-), CH2 group and other alkyl chains of the amino acids to V-2,m(0). The results on Delta(tr)V(2,m)(,)0 of amino acids from water to aqueous TX-100 solutions have been interpreted in terms of ion - ion, ion - polar, hydrophilic - hydrophilic and hydrophobic - hydrophobic group interactions. For all the six amino acids studied, the values of Delta(tr)V(2,m)(0) from water to all the studied concentrations of aqueous TX-100 are small in spite of their different hydrophobic content, indicating an overall balance in interactions of zwitterionic/ hydrophilic groups of amino acids with the hydrophilic groups of TX-100, and of hydrophobic and ionic/hydrophilic groups of the amino acids with hydrophobic groups of TX-100. Comparison of the interactions of the amino acids with nonionic, anionic and cationic surfactants has also been made and discussed. The partial specific volume of transfer of lysozyme from water to aqueous TX-100 solutions also indicates a balance of the hydrophobic and hydrophilic interactions in the protein - nonionic surfactant system.
 
Publisher PLENUM PUBLISHERS
 
Date 2011-08-27T06:21:47Z
2011-12-26T12:57:46Z
2011-12-27T05:45:10Z
2011-08-27T06:21:47Z
2011-12-26T12:57:46Z
2011-12-27T05:45:10Z
2004
 
Type Article
 
Identifier JOURNAL OF SOLUTION CHEMISTRY, 33(11), 1411-1427
0095-9782
http://dx.doi.org/10.1007/s10953-004-1056-x
http://dspace.library.iitb.ac.in/xmlui/handle/10054/11570
http://hdl.handle.net/10054/11570
 
Language en