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Interfacial properties as predictors of radioresistance in cervical cancer

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Title Interfacial properties as predictors of radioresistance in cervical cancer
 
Creator ANAND, PREETHA
HUILGOL, NAGRAJ
BANERJEE, RINTI
 
Subject surface tension
patient treatment
monolayers
hysteresis
 
Description The prediction of radioresistance of tumours, early in the course of radiotherapy, may help clinicians in deciding the optimal treatment strategy
for each case. This study was carried out to investigate an in vitro technique to predict radiosensitivity, after a single radiation dose of 2 Gy in
cervical cancer. Langmuir films of tissue homogenates of biopsy samples from 20 cervical cancer patients treated with radiotherapy alone and 15
normal controls were evaluated. The tensiometric profiles before and after giving 2 Gy of radiation, were compared with that of controls and were
correlated with the clinical radioresponsiveness evaluated on completion of the radiotherapy course of 70–78 Gy over a period of 50–55 days. The
tensiometric profiles measured after a single dose of radiation can be used to fingerprint the clinical radioresponsiveness of the cervical cancer
tissues. The hysteresis of the monolayers of completely radioresponsive post-radiotherapy tissue homogenates was 5.8 times greater than that of
partially radioresponsive post-radiotherapy tissue homogenates and was statistically significant using Mann–Whitney test (p < 0.05). From our
results, the following tensiometric criteria for prediction of radioresistance emerge. After first dose of radiation, if the minimum surface tension
of tissue homogenate is greater than 50 mN/m and hysteresis area is less than 20 μJ those tissues will be in the partially radioresponsive and for
completely radioresponsive tissue homogenates, the minimum surface tension will be less than 47 mN/m and the hysteresis area will be greater
than 33 μJ. The cholesterol and phospholipid content of radioresponsive cervical cancerous tissues after radiotherapy was found to be 1.2 and 2.2
times lower than that of the untreated tissues and due to lower lipid content organic phase surface activity of radioresponsive cancerous tissues
after radiotherapy was less than that of the untreated tissue organic phase. The radiation induced tensiometric profile changes of radioresponsive
cervical cancerous tissues can be correlated to the radiation induced lipid profile changes. This technique, due to its simplicity and high precision,
can serve as a predictive tool for radioresponsiveness and is easily translatable to the clinical setting. Randomized large sample trials are necessary
to validate this technique further and help in the translation from bench to clinics.
 
Publisher Elsevier
 
Date 2009-04-23T11:41:36Z
2011-12-08T06:25:22Z
2011-12-26T13:01:33Z
2011-12-27T05:45:12Z
2009-04-23T11:41:36Z
2011-12-08T06:25:22Z
2011-12-26T13:01:33Z
2011-12-27T05:45:12Z
2007
 
Type Article
 
Identifier Journal of Colloid and Interface Science 314(1), 63-70
0021-9797
10.1016/j.jcis.2007.04.011
http://hdl.handle.net/10054/1219
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1219
 
Language en