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Expression of GAL genes in a mutant strain of Saccharomyces cerevisiae lacking GAL80: quantitative model and experimental verification

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Title Expression of GAL genes in a mutant strain of Saccharomyces cerevisiae lacking GAL80: quantitative model and experimental verification
 
Creator VERMA, M
BHAT, PJ
VENKATESH, KV
 
Subject microbial-growth
yeast
amplification
fermentation
environment
repressor
systems
network
mig1
galactose
gal4p-binding sites
gal regulon
gene-expression dynamics
genetic switch
saccharomyces cerevisiae (baker's yeast)
 
Description The regulatory network of GAL genes is a model system for the production of foreign proteins. A mathematical model based on steady state was developed for the expression of GAL (galactosidase) genes in a mutant strain of Saccharomyces cerevisiae lacking GAL80. The transcriptional and translational responses of the GAL switch were predicted at various steady-state glucose concentrations. The model predicted ultrasensitive transcriptional response with a Hill coefficient (h) of 1.9 and 3.2 for genes with one and two binding sites respectively. Further, a lesser degree of ultrasensitivity was predicted for translational response with an h value of 1.3 for genes with one binding site and 2.1 for genes with two binding sites. The ultrasensitivity was due to dimerization of regulatory protein Gal4p and co-operative binding of Gal4p to DNA. The steady-state predictions were experimentally verified through measurements of a-galactosidase (for one binding site) and beta-galactosidase (for two binding sites). The steady state model was further extended to represent the dynamic expression profile and the same was verified experimentally. The growth phase and the synthesis of foreign protein could be distinctly separated using a mutant strain of Soccharomyces cerevisiae (baker's yeast).
 
Publisher PORTLAND PRESS
 
Date 2011-08-27T06:47:05Z
2011-12-26T12:57:47Z
2011-12-27T05:45:13Z
2011-08-27T06:47:05Z
2011-12-26T12:57:47Z
2011-12-27T05:45:13Z
2004
 
Type Article
 
Identifier BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 39(), 89-97
0885-4513
http://dx.doi.org/10.1042/BA20030119
http://dspace.library.iitb.ac.in/xmlui/handle/10054/11576
http://hdl.handle.net/10054/11576
 
Language en