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Steady-state analysis of glucose repression reveals hierarchical expression of proteins under Mig1p control in Saccharomyces cerevisiae

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Title Steady-state analysis of glucose repression reveals hierarchical expression of proteins under Mig1p control in Saccharomyces cerevisiae
 
Creator VERMA, M
BHAT, PJ
VENKATESH, KV
 
Subject yeast gal genes
catabolite repression
binding
switch
activator
promoter
growth
model
transcription
sensitivity
glucose repression
mig1p
mitogen-activated protein kinase (mapk)
transcriptional activator
transcriptional repressor
yeast
 
Description Glucose repression is a global transcriptional regulatory mechanism commonly observed in micro-organisms for the repression of enzymes that are not essential for glucose metabolism. In Saccharomyces cerevisiae, Mig1p, a homologue of Wilms' tumour protein, is a global repressor protein dedicated to glucose repression. Mig1p represses genes either by binding directly to the upstream repression sequence of structural genes or by indirectly repressing a transcriptional activator, such as Ga14p. In addition, some genes are repressed by both of the above mechanisms. This raises a fundamental question regarding the physiological relevance of the varied mechanisms of repression that exist involving Mig1p. We address this issue by comparing two well-known glucose-repression systems, that is, SUC2 and GAL gene expression systems, which encompass all the above three mechanisms. We demonstrate using steady-state analysis that these mechanisms lead to a hierarchical glucose repression profile of different family of genes. This switch over from one carbon source to another is well-calibrated as a function of glucose concentration through this hierarchical transcriptional response. The mechanisms prevailing in this repression system can achieve amplification and sensitivity, as observed in the well-characterized MAPK (mitogen-activated protein kinase) cascade system, albeit through a different structure. A critical feature of repression predicted by our steady-state model for the mutant strain of S. cerevisiae lacking Gal80p agrees well with the data reported here as well as that available in the literature.
 
Publisher PORTLAND PRESS LTD
 
Date 2011-08-27T07:10:58Z
2011-12-26T12:57:47Z
2011-12-27T05:45:16Z
2011-08-27T07:10:58Z
2011-12-26T12:57:47Z
2011-12-27T05:45:16Z
2005
 
Type Article
 
Identifier BIOCHEMICAL JOURNAL, 388(), 843-849
0264-6021
http://dx.doi.org/10.1042/BJ20041883
http://dspace.library.iitb.ac.in/xmlui/handle/10054/11581
http://hdl.handle.net/10054/11581
 
Language en