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Concanavalin A modulates tyrosine phosphorylation and activation of a type II phosphatidylinositol 4-kinase in rat splenic lymphocytes

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Title Concanavalin A modulates tyrosine phosphorylation and activation of a type II phosphatidylinositol 4-kinase in rat splenic lymphocytes
 
Creator FERNANDIS, AZ
SUBRAHMANYAM, G
 
Subject t-cell activation
antigen receptor
signal-transduction
phosphoinositide kinases
protein
purification
stimulation
turnover
pathways
binding
t-cell activation
signal transduction
 
Description Activation of rat splenic lymphocytes by concanavalin A resulted in two-fold increase in PtdIns 4-kinase activity and rapid tyrosine phosphorylation of the enzyme. The activation kinetics showed a strong correlation with tyrosine phosphorylation state of the enzyme. Characterization of the enzyme activity suggests that it is a type II PtdIns 4-kinase. Kinetic analysis of the enzyme reaction showed three-fold decrease in K-m for PtdIns and two-fold increase in V-max in Con A stimulated cells. These results suggest that a type II PtdIns 4-kinase is an integral component of the early signal transduction machinery during T-cell activation by concanavalin A and is actively regulated by protein tyrosine phosphorylation-dephosphorylation. (C) 1998 . .
 
Publisher PERGAMON-ELSEVIER SCIENCE LTD
 
Date 2011-08-23T20:32:58Z
2011-12-26T12:56:35Z
2011-12-27T05:45:32Z
2011-08-23T20:32:58Z
2011-12-26T12:56:35Z
2011-12-27T05:45:32Z
1998
 
Type Article
 
Identifier MOLECULAR IMMUNOLOGY, 35(14-15), 935-941
0161-5890
http://dx.doi.org/10.1016/S0161-5890(98)00078-9
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10635
http://hdl.handle.net/10054/10635
 
Language en