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Effects of pH and ionic strength on the assembly and bundling of FtsZ protofilaments: a possible role of electrostatic interactions in the bundling of protofilaments

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Title Effects of pH and ionic strength on the assembly and bundling of FtsZ protofilaments: a possible role of electrostatic interactions in the bundling of protofilaments
 
Creator BEURIA, TUSHAR K
SHAH, JAIMIN H
SANTRA, MANAS KUMAR
KUMAR, VINAY
PANDA, DULAL
 
Subject assembly
circular dichroism
stability criteria
monomers
electrostatic
 
Description Assembly, bundling and stability of FtsZ protofilaments are important for the formation and functioning of the cytokinetic Z-ring during bacterial
division. We found that the bundling of FtsZ protofilaments decreased strongly with increasing pH from 6.0 to 7.9, while the assembly of FtsZ
monomers did not decrease considerably. In addition, the disassembly of FtsZ protofilaments was strongly suppressed at pH 6.0 as compared to
the elevated pHs. The far-UV circular dichroism spectra of the native FtsZ and the tryptophan emission spectra of mutated FtsZ (Y371W) did not
change by increasing pH from 6 to 7.9 indicating that the structure of FtsZ was not altered significantly. Further, the inhibition of bundling of FtsZ
protofilaments predominantly, and the inhibition of assembly to a lesser extent by salt indicated that electrostatic interactions are important for
the assembly and bundling of FtsZ protofilaments. These observations are supported by the results of computational docking of Escherichia coli
dimer structure. The results suggest that the basic intracellular pH (7.4–7.8) of E. coli may play a role in regulating the assembly dynamics of FtsZ
in the Z-ring by reducing protofilament stability and bundling in bacterial cytoplasm.
 
Publisher Elsevier
 
Date 2009-04-24T09:13:22Z
2011-12-08T06:28:26Z
2011-12-26T13:01:38Z
2011-12-27T05:45:39Z
2009-04-24T09:13:22Z
2011-12-08T06:28:26Z
2011-12-26T13:01:38Z
2011-12-27T05:45:39Z
2006
 
Type Article
 
Identifier International Journal of Biological Macromolecules 40(1), 30-39
0141-8130
10.1016/j.ijbiomac.2006.05.006
http://hdl.handle.net/10054/1235
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1235
 
Language en