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Effects of cooling and ARL 67156 on synaptic ecto-ATPase activity in guinea pig and mouse vas deferens

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Title Effects of cooling and ARL 67156 on synaptic ecto-ATPase activity in guinea pig and mouse vas deferens
 
Creator GHILDYAL, PARA
MANCHANDA, R
 
Subject enzyme activity
modulation
hydrolysis
temperature
 
Description We have studied the influence of temperature and ARL 67156 on ATP hydrolysis in mouse and guinea pig vas deferens in order to explore the properties of the enzymatic inactivation mechanism proposed to regulate purinergic neurotransmission at the sympathetic neuromuscular junction of smooth muscle. The ectonucleotidase activity was determined by using the malachite green method to measure the inorganic phosphate (Pi) liberated with ATP used as a substrate. ATP hydrolysis in both species was found to be insensitive to ouabain (100 μM), sodium azide (1 mM), sodium vanadate (100 μM) and β-glycerophosphate (10 mM) and was also found to depend on Ca2+ and Mg2+. VMAX of the ectonucleotidase activity for guinea pig and mouse vas deferens was 958.4±66.3 and 79.7±8.5 pmol/min/mg, while KM was 625.1±45.2 and 406.0±29.0 μM, respectively. Cooling the tissues from 35 to 25 °C reduced the enzyme activity significantly (P
 
Publisher Elsevier
 
Date 2009-04-24T09:15:10Z
2011-12-08T06:30:57Z
2011-12-26T13:01:39Z
2011-12-27T05:45:46Z
2009-04-24T09:15:10Z
2011-12-08T06:30:57Z
2011-12-26T13:01:39Z
2011-12-27T05:45:46Z
2004
 
Type Article
 
Identifier Autonomic Neuroscience 115(1-2), 28-34
1566-0702
10.1016/j.autneu.2004.07.005
http://hdl.handle.net/10054/1240
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1240
 
Language en