Record Details

Depth-dependent analysis of membranes using benzophenone-based phospholipids

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Depth-dependent analysis of membranes using benzophenone-based phospholipids
 
Creator JOHN, B
KUMAR, ER
LALA, AK
 
Subject inter-molecular crosslinking
fatty acyl chains
hydrophobic core
bilayers
phosphatidylcholine
fluorescence
reagent
acid
depth-dependent analysis
cross-linking
benzophenone-based phospholipids
gaussian
 
Description Any attempt to probe the membrane hydrophobic core with chemical reagents necessitates the use of reactive intermediates like carbenes and nitrenes, which can insert into C-H bonds. Several photoactivable reagents based on carbenes and nitrenes have been reported. However, the high reactivity of these reagents, often leads to very low insertion yields. We report here a high degree of cross-linking (35-40%) achieved with three benzophenone-based phospholipids and analyze the carbon functionalization data using a multiple Gaussian function. These phospholipids are so designed so as to permit depth-dependent labeling in membranes. Single bilayer vesicles were prepared from these phospholipids and dimyristoylphosphatidylcholine. The cross-linked product was isolated and characterized by mass spectroscopy. The results obtained indicated that the cross-linked product was dominated by dimeric product formed by intermolecular cross-linking. The Gaussian analysis used here provides insight into the relative depths of the probes inside the membrane. (C) 2000 .
 
Publisher ELSEVIER SCIENCE BV
 
Date 2011-07-24T17:11:16Z
2011-12-26T12:47:23Z
2011-12-27T05:45:54Z
2011-07-24T17:11:16Z
2011-12-26T12:47:23Z
2011-12-27T05:45:54Z
2000
 
Type Article
 
Identifier BIOPHYSICAL CHEMISTRY, 87(1), 37-42
0301-4622
http://dx.doi.org/10.1016/S0301-4622(00)00178-2
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6486
http://hdl.handle.net/10054/6486
 
Language en