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Competitive inhibition of glutamate dehydrogenase reaction

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Title Competitive inhibition of glutamate dehydrogenase reaction
 
Creator CHOUDHURY, R
PUNEKAR, NS
 
Subject nicotinamide-adenine dinucleotide
molecular-interactions
aspergillus-nidulans
alpha-ketoglutarate
kinetic mechanism
purification
acid
analogs
glutamate dehydrogenase
2-methyleneglutarate
methacrylate
competitive inhibitors
 
Description Irrespective of their pyridine nucleotide specificity, all glutamate dehydrogenases; share a common chemical mechanism that involves an enzyme bound 'iminoglutarate' intermediate. Three compounds, structurally related to this intermediate, were tested for the inhibition of purified NADP-glutamate dehydrogenases from two Aspergilli, as also the bovine liver NAD(P)-glutamate dehydrogenase. 2-Methyleneglutarate, closely resembling iminoglutarate, was a potent competitive inhibitor of the glutamate dehydrogenase reaction. This is the first report of a non-aromatic structure with a better glutamate dehydrogenase inhibitory potency than aryl carboxylic acids such as isophthalate. A suitably located 2-methylene group to mimic the iminium ion could be exploited to design inhibitors of other amino acid dehydrogenases. (c) 2007 on behalf of the Federation of European Biochemical Societies.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2011-07-24T13:55:54Z
2011-12-26T12:56:55Z
2011-12-27T05:46:28Z
2011-07-24T13:55:54Z
2011-12-26T12:56:55Z
2011-12-27T05:46:28Z
2007
 
Type Article
 
Identifier FEBS LETTERS, 581(14), 2733-2736
0014-5793
http://dx.doi.org/10.1016/j.febslet.2007.05.032
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6444
http://hdl.handle.net/10054/6444
 
Language en