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Glucose response of dissolved-core alginate microspheres: towards a continuous glucose biosensor

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Title Glucose response of dissolved-core alginate microspheres: towards a continuous glucose biosensor
 
Creator CHAUDHARY, A
MCSHANE, MJ
SRIVASTAVA, R
 
Subject energy-transfer assay
in-vivo
affinity sensor
blood-glucose
concanavalin-a
polyelectrolyte microcapsules
macromolecule encapsulation
dexamethasone release
nanofilm coatings
binding
 
Description Microparticle optical sensors hold potential as implantable smart materials for in vivo analysis. In this work, the reversible response of dissolved-core alginate microspheres containing a homogeneous fluorescence resonance energy transfer (FRET)-based competitive binding assay for glucose was evaluated. The layer-by-layer self assembly technique was used to deposit multilayered nanofilm coatings on the alginate microspheres containing the assay, thereby stabilizing the sensor system when the alginate was de-crosslinked. The response to glucose was then determined in DI water and simulated interstitial fluid (SIF) using a flow cell to establish controlled, dynamic flow conditions for demonstrating reversibility. The glucose sensitivity under dynamic conditions was estimated to be 0.52%/mM glucose in DI water and 0.6%/mM glucose in simulated interstitial fluid; in both cases, the analytical response range was 0-30mM glucose, covering both physiological (normoglycemia) and pathophysiological range (hyperglycemia and hypoglycemia). The sensor demonstrated a repeatable and reproducible response when tested over a period of one month, under dynamic flow conditions. Finally, in vitro cytotoxicity assays performed with L929 mouse fibroblast cell lines suggested that the dissolved-core alginate microsphere sensor system with nanofilm coating has sufficient biocompatibility for use as implantable glucose biosensors.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2011-08-28T11:27:17Z
2011-12-26T12:58:01Z
2011-12-27T05:46:54Z
2011-08-28T11:27:17Z
2011-12-26T12:58:01Z
2011-12-27T05:46:54Z
2010
 
Type Article
 
Identifier ANALYST, 135(10), 2620-2628
0003-2654
http://dx.doi.org/10.1039/c0an00109k
http://dspace.library.iitb.ac.in/xmlui/handle/10054/11728
http://hdl.handle.net/10054/11728
 
Language en