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Immobilization of antibodies on polyaniline films and its application in a piezoelectric immunosensor

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Title Immobilization of antibodies on polyaniline films and its application in a piezoelectric immunosensor
 
Creator SAI, VVR
MAHAJAN, S
CONTRACTOR, AQ
MUKHERJI, S
 
Subject electrodes
monolayers
biosensors
composite
polymers
sensor
 
Description Conducting polymers, especially polyaniline (PAni), have been extensively used in biosensor applications. A protocol for covalent immobilization of human IgG on polyaniline using glutaraldehyde as the cross-linker is described in this report and utilized in development of a piezoelectric immunosensor. Here, PAni was used as the substrate for immobilization. The electropolymerization parameters were optimized to get suitable thickness and surface morphology of the PAni for obtaining high density and uniformity of immobilized antibodies on the surface of our films. Possible reaction between PAni thin films and glutaraldehyde was explored using FT-IR characterization in grazing angle mode and XPS. The protocol has been characterized with the help of quartz crystal microbalance analysis. An antibody surface density of 4.86 ng/ mm(2) was obtained. A piezoelectric biosensor developed for detection of IgG with the proposed protocol was capable of differentiating the target analyte concentrations between 500 ng/ mL and 25 mu g/mL with nonspecific binding of similar to 10%.
 
Publisher AMER CHEMICAL SOC
 
Date 2011-07-14T07:29:06Z
2011-12-26T12:48:15Z
2011-12-27T05:47:29Z
2011-07-14T07:29:06Z
2011-12-26T12:48:15Z
2011-12-27T05:47:29Z
2006
 
Type Article
 
Identifier ANALYTICAL CHEMISTRY, 78(24), 8368-8373
0003-2700
http://dx.doi.org/10.1021/ac060120a
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3895
http://hdl.handle.net/10054/3895
 
Language en