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Improvement in enzyme productivities from mold cultivations using the liquid-phase oxygen supply strategy

DSpace at IIT Bombay

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Title Improvement in enzyme productivities from mold cultivations using the liquid-phase oxygen supply strategy
 
Creator RAWOOL, SB
SAHOO, S
RAO, KK
SURESHKUMAR, GK
 
Subject hydrogen-peroxide
xanthomonas-campestris
cultures
pellets
 
Description Cultivations of Aspergillus niger cells in which oxygen was provided through the liquid-phase oxygen supply strategy (which involves the need-based decomposition of H2O2 pulses to yield the necessary oxygen) were studied. Concentrations of H2O2 in the range of 1 to 5 mM were found to be suitable for use in cultivations. The growth rate constant of 0.17 h(-1) obtained in the H2O2-based cultivation, was comparable to that obtained in the cultivation with aeration; however, the maximum cell concentration in the H2O2-based cultivation was 124% of that obtained in cultivation with aeration. Maximum concentrations of catalase, protease and glucose oxidase obtained in the H2O2-based cultivation, were 240%, 172%, and 124% respectively, of those obtained in the cultivation with aeration. Further, the specific enzyme levels (units per gram cell) of catalase and protease obtained in the H2O2-based cultivation were 172% and 156% of those obtained in the cultivation with aeration, whereas, the specific glucose oxidase levels were comparable. In addition, the oxygen profiles inside the pellets of A. niger during both the modes of oxygen supply were described using mathematical models. Studies to elucidate the mechanism of oxygen availability showed that the decomposition of the H2O2 supplied in the extracellular space occurred intracellularly, as well as extracellularly, to yield oxygen. Also, the proton motive force (PMF) was found to be involved in the process of oxygen availability from H2O2 to A. niger cells.
 
Publisher AMER CHEMICAL SOC
 
Date 2011-07-14T07:47:22Z
2011-12-26T12:48:16Z
2011-12-27T05:47:40Z
2011-07-14T07:47:22Z
2011-12-26T12:48:16Z
2011-12-27T05:47:40Z
2001
 
Type Article
 
Identifier BIOTECHNOLOGY PROGRESS, 17(5), 832-837
8756-7938
http://dx.doi.org/10.1021/bp010078z
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3899
http://hdl.handle.net/10054/3899
 
Language en