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Biodegradation of mixture containing monohydroxybenzoate isomers by Acinetobacter calcoaceticus

DSpace at IIT Bombay

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Title Biodegradation of mixture containing monohydroxybenzoate isomers by Acinetobacter calcoaceticus
 
Creator PRATHIBHA, K
SUMATHI, S
 
Subject p-hydroxybenzoic acid
salicylate hydroxylase
3-hydroxybenzoate 6-hydroxylase
pseudomonas-putida
rhodococcus-erythropolis
protocatechuic acid
beta-ketoadipate
strain u2
purification
degradation
acinetobacter calcoaceticus
2-hydroxybenzoic acid
3-hydroxybenzoic acid
4-hydroxybenzoic acid
oxygenases
 
Description A bacterial strain capable of utilizing a mixture containing 2-hydroxybenzoic acid (2-HBA), 3-hydroxybenzoic acid (3-HBA) and 4-hydroxybenzoic (4-HBA) acid was isolated through enrichment from a soil sample. Based on 16SrDNA sequencing, the microorganism was identified as Acinetobacter calcoaceticus. The sequence of biodegradation of the three isomers when provided as a mixture (0.025%, w/v each) was elucidated. The dihydroxylated metabolites formed from the degradation of 2-HBA, 3-HBA and 4-HBA were identified as catechol, gentisate and protocatechuate, respectively, using the cell-free supernatant and cell-free crude extracts. Monooxygenases and dioxygenases that were induced in the cells of Acinetobacter calcoaceticus in response to growth on mixture containing 2-HBA, 3-HBA and 4-HBA could be detected in cell-free extracts. These data revealed the pathways operating in Acinetobacter calcoaceticus for the sequential metabolism of monohydroxybenzoate isomers when presented as a mixture.
 
Publisher SPRINGER
 
Date 2011-08-29T10:12:58Z
2011-12-26T12:58:32Z
2011-12-27T05:48:41Z
2011-08-29T10:12:58Z
2011-12-26T12:58:32Z
2011-12-27T05:48:41Z
2008
 
Type Article
 
Identifier WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 24(6), 813-823
0959-3993
http://dx.doi.org/10.1007/s11274-007-9545-x
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12051
http://hdl.handle.net/10054/12051
 
Language en