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Conjugative transfer of preferential utilization of aromatic compounds from Pseudomonas putida CSV86

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Title Conjugative transfer of preferential utilization of aromatic compounds from Pseudomonas putida CSV86
 
Creator BASU, A
PHALE, PS
 
Subject mobile genetic elements
sp. strain b13
genomic islands
plasmid dna
naphthalene
bacteria
pathway
biodegradation
integration
metabolism
conjugative element
pseudomonas putida csv86
biodegradation
carbon source preference
naphthalene and methylnaphthalene metabolism
 
Description Pseudomonas putida CSV86 utilizes naphthalene (Nap), salicylate (Sal), benzyl alcohol (Balc), and methylnaphthalene (MN) preferentially over glucose. Methylnaphthalene is metabolized by ring-hydroxylation as well as side-chain hydroxylation pathway. Although the degradation property was found to be stable, the frequency of obtaining Nap(-)Sal(-)MN(-)Balc(-) phenotype increased to 11% in the presence of curing agents. This property was transferred by conjugation to Stenotrophomonas maltophilia CSV89 with a frequency of 7 x 10(-8) per donor cells. Transconjugants were Nap(+)Sal(+)MN(+)Balc(+) and metabolized MN by ring- as well as side-chain hydroxylation pathway. Transconjugants also showed the preferential utilization of aromatic compounds over glucose indicating transfer of the preferential degradation property. The transferred properties were lost completely when transconjugants were grown on glucose or 2YT. Attempts to detect and isolate plasmid DNA from CSV86 and transconjugants were unsuccessful. Transfer of degradation genes and its subsequent loss from the transconjugants was confirmed by PCR using primers specific for 1,2-dihydroxynaphthalene dioxygenase and catechol 2,3-dioxygenase (C23O) as well as by DNA-DNA hybridizations using total DNA as template and C23O PCR fragment as a probe. These results indicate the involvement of a probable conjugative element in the: (i) metabolism of aromatic compounds, (ii) ring- and side-chain hydroxylation pathways for MN, and (iii) preferential utilization of aromatics over glucose.
 
Publisher SPRINGER
 
Date 2011-08-29T10:56:31Z
2011-12-26T12:58:33Z
2011-12-27T05:48:44Z
2011-08-29T10:56:31Z
2011-12-26T12:58:33Z
2011-12-27T05:48:44Z
2008
 
Type Article
 
Identifier BIODEGRADATION, 19(1), 83-92
0923-9820
http://dx.doi.org/10.1007/s10532-007-9117-7
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12061
http://hdl.handle.net/10054/12061
 
Language en