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Effects of carbenoxolone on syncytial electrical properties and junction potentials of guinea-pig vas deferens

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Title Effects of carbenoxolone on syncytial electrical properties and junction potentials of guinea-pig vas deferens
 
Creator PALANI, D
GHILDYAL, P
MANCHANDA, R
 
Subject smooth-muscle-cells
synaptic potentials
gap-junctions
current flow
varicosities
communication
transmission
oscillations
contractile
secretion
guinea-pig vas deferens
gap junction
carbenoxolone
smooth muscle
cell-to-cell coupling
 
Description The effects of the putative gap junction blocker carbenoxolone on smooth muscle syncytial properties and junction potentials were studied in guinea pig vas deferens (GPVD). Treatment with 50 mu M carbenoxolone reversibly and significantly increased input resistance (R-in) ( by 682.5 +/- 326.0 %, P < 0.05) and abolished cable potentials within 6 7 mins of incubation, without disturbing resting membrane potential. Carbenoxolone reversibly and significantly increased the amplitude of spontaneous excitatory junction potentials (sEJPs) by 96.9 +/- 35.45% (P < 0.05), shifted their amplitude distribution rightwards, and reduced their frequency of occurrence by 58.17 +/- 17.7% (P < 0.05), without altering their time courses. Similarly, carbenoxolone increased the amplitude of evoked excitatory junction potentials (eEJPs) by 17.7 +/- 5.88% and tau(decay) by 19.43 +/- 8.29% (P < 0.05). Our results indicate that carbenoxolone alters the electrical properties and junctional potentials of the GPVD by a mechanism consistent with a relatively specific block of gap junctions. These results suggest that gap junction mediated cell-to-cell communication may significantly modulate the electrical properties and junctional potentials of the GPVD and consequently the physiological functioning of this tissue.
 
Publisher SPRINGER
 
Date 2011-08-29T12:30:40Z
2011-12-26T12:58:35Z
2011-12-27T05:48:47Z
2011-08-29T12:30:40Z
2011-12-26T12:58:35Z
2011-12-27T05:48:47Z
2006
 
Type Article
 
Identifier NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 374(3), 207-214
0028-1298
http://dx.doi.org/10.1007/s00210-006-0109-7
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12082
http://hdl.handle.net/10054/12082
 
Language en