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LOCATION OF LYSINE-129 AND LYSINE-40 41 WITH RESPECT TO RETINYLIDENE CHROMOPHORE IN BACTERIORHODOPSIN

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Title LOCATION OF LYSINE-129 AND LYSINE-40 41 WITH RESPECT TO RETINYLIDENE CHROMOPHORE IN BACTERIORHODOPSIN
 
Creator SONAR, SM
SINGH, AK
 
Subject purple membrane
vibrational spectroscopy
sequencing methods
energy-transfer
amino-acids
protein
photocycle
mutants
bacteriorhodopsin
chemical modification
fluorescamine
fluorescence
forster resonance energy transfer
(h-halobium)
 
Description Fluorescence properties of fluorescamine-modified bacteriorhodopsin (BR) have been studied. BR reacts with fluorescamine (1:2 ratio) to give protein (FLBR-I) modified at Lys-129. By making use of citraconic anhydride as a masking reagent, fluorescamine modification of Lys-40/41 in BR has been achieved. Forster's resonance energy transfer studies indicate that the distance between FL-Lys-129 and retinylidene chromophore is 11 angstrom, whereas that between FL-Lys-40/41 and retinylidene chromophore is 24 angstrom. These measured distances have been analysed in terms of BR structure.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2011-07-25T15:47:55Z
2011-12-26T12:51:21Z
2011-12-27T05:48:53Z
2011-07-25T15:47:55Z
2011-12-26T12:51:21Z
2011-12-27T05:48:53Z
1991
 
Type Article
 
Identifier BIOCHIMICA ET BIOPHYSICA ACTA, 1076(2), 239-244
0006-3002
http://dx.doi.org/10.1016/0167-4838(91)90273-3
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6724
http://hdl.handle.net/10054/6724
 
Language en