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Interactions between hematological derivatives and dipalmitoyl phosphatidyl choline: implications for adult respiratory distress syndrome

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Title Interactions between hematological derivatives and dipalmitoyl phosphatidyl choline: implications for adult respiratory distress syndrome
 
Creator RACHANA
BANERJEE, R
 
Subject blood
monolayers
cell
surface active agents
 
Description Blood and its components flood the alveoli in adult respiratory distress syndrome (ARDS) and may be responsible for the inhibition of lung surfactant in this syndrome. We have evaluated the surface properties of dipalmitoyl phosphatidylcholine (DPPC) monolayers - the main component of lung surfactant, in the presence of blood and its components. Experiments were performed using a Langmuir-Blodgett trough at physiological temperature (37°C), pH 7.0 and using 0.9% saline as the sub-phase. Whole blood (WB), membranes obtained from whole blood cells (Mem), lysed blood (LB), homogenized blood clot (CLOT), serum (SER), platelet rich plasma (PRP), platelet poor plasma (PPP) and individual plasma proteins (albumin, fibrinogen) were added to the sub-phase in the equivalent proportion of 10 parts of DPPC per million parts (w/v) of hematological inhibitor. Cell membranes were found to be the most inhibitory agent for DPPC surface activity as evidenced by an increase in the minimum surface tension (from 0.818±0.219 to 7.373±0.854mN/m) and percentage area change required to reduce the surface tension from 30 to 10mN/m (from 21.24±0.99 to 66.83±4.44). The inhibitory potential of pure plasma proteins differed from those of more complex blood derivatives like platelet rich plasma and serum. Whole blood and platelet poor plasma were non-inhibitory, but serum, platelet rich plasma and clot significantly increased the minimum surface tension of DPPC to 6.819±0.925, 6.625±2.261 and 6.060±0.640mN/m, respectively. These results were statistically significant with one-way analysis of variance and Newman-Keul's test (P
 
Publisher Elsevier
 
Date 2009-06-22T04:44:18Z
2011-12-08T08:01:11Z
2011-12-26T13:02:34Z
2011-12-27T05:49:02Z
2009-06-22T04:44:18Z
2011-12-08T08:01:11Z
2011-12-26T13:02:34Z
2011-12-27T05:49:02Z
2004
 
Type Article
 
Identifier Colloids and Surfaces B: Biointerfaces 34(2), 95-104
09277765
10.1016/j.colsurfb.2003.12.001
http://hdl.handle.net/10054/1559
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1559
 
Language en