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Multiple copies of MRG19 suppress transcription of the GAL1 promoter in a GAL80-dependent manner in Saccharomyces cerevisiae

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Title Multiple copies of MRG19 suppress transcription of the GAL1 promoter in a GAL80-dependent manner in Saccharomyces cerevisiae
 
Creator KABIR, MA
KHANDAY, FA
MEHTA, DV
BHAT, PJ
 
Subject carbon catabolite repression
regulatory gene gal80
snf1 protein-kinase
glucose repression
gal4p-activated genes
galactose induction
activator protein
stationary-phase
mig1 repressor
plasmid dna
gal regulon
transcription
saccharomyces cerevisiae
galactose suppression
 
Description A plasmid clone that suppresses galactose toxicity in a gal7 yeast strain has been isolated from a multicopy genomic DNA library. Molecular analysis revealed that the region responsible for the suppression of galactose toxicity corresponds to the ORF YPR030w, which was named MRG19. A CEN-based plasmid carrying the above ORF was unable to suppress the toxicity. Galactokinase activity was substantially reduced in cell extracts obtained from transformants bearing multiple copies of MRG19. Multiple copies of MRG19 were also able to suppress galactokinase expression driven by the CYC1 promoter but not the TEF1 promoter. Multiple copies of MRG19 could not suppress GAL1-driven galactokinase expression in a gal80 strain. However, MRG19-mediated suppression of CYC1-driven galactokinase expression was independent of GAL80 function. These results imply that multiple copies of MRG19 suppress galactokinase expression probably at the level of transcription. In agreement with this idea, multiple copies of MRG19 also suppress B-galactosidase expression driven by the GAL1 promoter in a GAL80-dependent manner. Disruption of MRG19 leads to an increase in the cell density at stationary phase in synthetic complete medium. MRG19 encodes a previously uncharacterised 124-kDa protein that shows no sequence homology to any known proteins.
 
Publisher SPRINGER VERLAG
 
Date 2011-08-30T09:31:59Z
2011-12-26T12:58:52Z
2011-12-27T05:49:32Z
2011-08-30T09:31:59Z
2011-12-26T12:58:52Z
2011-12-27T05:49:32Z
2000
 
Type Article
 
Identifier MOLECULAR AND GENERAL GENETICS, 262(6), 1113-1122
0026-8925
http://dx.doi.org/10.1007/PL00008654
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12267
http://hdl.handle.net/10054/12267
 
Language en