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Mica Surface Promotes the Assembly of Cytoskeletal Proteins

DSpace at IIT Bombay

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Title Mica Surface Promotes the Assembly of Cytoskeletal Proteins
 
Creator HAMON, L
PANDA, D
SAVARIN, P
JOSHI, V
BERNHARD, J
MUCHER, E
MECHULAM, A
CURMI, PA
PASTRE, D
 
Subject bacterial-cell division
atomic-force microscopy
ftsz
dna
tubulin
polymerization
visualization
degradation
hydrolysis
dynamics
 
Description We report the surface-mediated polymerization of FtsZ protein, the prokaryote homologue of tubulin, by AFM. FtsZ protein can form filaments on mica whereas the bulk FtsZ concentration is orders of magnitude lower than the critical concentration. Surface polymerization is favored by a local increase in protein concentration and requires a high mobility of proteins on the surface. To generalize to other cytoskeleton protein, we also show that mica can initiate the formation of tubulin protofilaments. This study is of particular interest for studying cytoskeletal protein dynamics by AFM but also for the surface autoassembly of nanostructures.
 
Publisher AMER CHEMICAL SOC
 
Date 2011-07-14T11:23:44Z
2011-12-26T12:48:21Z
2011-12-27T05:49:40Z
2011-07-14T11:23:44Z
2011-12-26T12:48:21Z
2011-12-27T05:49:40Z
2009
 
Type Article
 
Identifier LANGMUIR, 25(6), 3331-3335
0743-7463
http://dx.doi.org/10.1021/la8035743
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3940
http://hdl.handle.net/10054/3940
 
Language en