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Stabilization of Sensing Assay within Polyelectrolyte-Coated Alginate Microspheres for Optical Urea Sensing

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Title Stabilization of Sensing Assay within Polyelectrolyte-Coated Alginate Microspheres for Optical Urea Sensing
 
Creator SWATI, M
SRIVASTAVA, R
 
Subject layered double hydroxides
enzyme immobilization
biosensor
sensor
ph
microcapsules
membranes
matrices
release
ammonia
alginate
enzyme immobilization
urea sensing
 
Description A new absorbance-based enzymatic biosensor for determination of urea (in the range 0.01 to 6.7 mM) is described. Quantification using cresol red dye, immobilized in the nanofilm coatings assembled on alginate microspheres to immobilize the urease enzyme, has been accomplished using ratiometric absorbance measurements. The effect of salt concentration in polyelectrolyte nanofilms (on the stability of dye molecules) and buffer pH (on the enzyme stability) are reported. The results demonstrate excellent stability of sensing assay within alginate microspheres. Urea-sensing experiments demonstrate the potential to develop an optical urea sensor that is stable over a month.
 
Publisher TAYLOR & FRANCIS INC
 
Date 2011-08-30T18:41:23Z
2011-12-26T12:59:07Z
2011-12-27T05:50:03Z
2011-08-30T18:41:23Z
2011-12-26T12:59:07Z
2011-12-27T05:50:03Z
2009
 
Type Article
 
Identifier ANALYTICAL LETTERS, 42(5), 790-806
0003-2719
http://dx.doi.org/10.1080/00032710802677175
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12425
http://hdl.handle.net/10054/12425
 
Language en