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Photocleavage of plasmid DNA by the prophyrin meso-tetrakis[4-(carboxymethyleneoxy)phenyl]porphyrin

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Title Photocleavage of plasmid DNA by the prophyrin meso-tetrakis[4-(carboxymethyleneoxy)phenyl]porphyrin
 
Creator CHATTERJEE, SR
SHETTY, SJ
DEVASAGAYAM, TPA
SRIVASTAVA, TS
 
Subject single-stranded-dna
circular-dichroism
porphyrin
oxygen
binding
intercalation
constants
complexes
cleavage
charges
porphyrin
pbr322 dna
photocleavage
single stand breaks
singlet oxygen
soret band
circular dichroism
fluorescence
energy transfer
 
Description meso-Tetrakis [4-(carboxymethylenzoxy)phenyl]porphyrin (H(2)T4CPP) cleaves pBR322 plasmid DNA to single strand breaks in the presence of molecular oxygen and visible light. The above photocleavage was much more efficient in D2O buffer of sodium phosphate (pD = 7.4) than H2O buffer of sodium phosphate (pH = 7.4). In addition this photocleavage of plasmid DNA was inhibited in the presence of sodium azide, lipoic acid, tert-butanol or mannitol suggesting the involvement of O-1(2) and OH in the photocleavage of plasmid DNA. The photocleavage was observed to be more efficient in the presence of H2CPP than in the presence of H(2)T4CPP [meso-tetrakis(4-carboxy-phenyl) porphyrin]. Our spectral studies using UV-visible, fluorescence and circular dichroism techniques suggest that H(2)T4CPP binds to DNA while H(2)T4CPP does not, Thus, the difference in photocleavage may be caused by the nonbinding of H2CPP and by the binding of H(2)T4CPP to calf thymus (CT) DNA. (C) 1997 Elsevier Science S.A.
 
Publisher ELSEVIER SCIENCE SA
 
Date 2011-07-28T16:16:43Z
2011-12-26T13:02:52Z
2011-12-27T05:50:08Z
2011-07-28T16:16:43Z
2011-12-26T13:02:52Z
2011-12-27T05:50:08Z
1997
 
Type Article
 
Identifier JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 41(1-2), 128-135
1011-1344
http://dx.doi.org/10.1016/S1011-1344(97)00094-8
http://dspace.library.iitb.ac.in/xmlui/handle/10054/7445
http://hdl.handle.net/10054/7445
 
Language en