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Interaction of 3-hydroxybenzoate-6-hydroxylase with cibacron blue

DSpace at IIT Bombay

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Title Interaction of 3-hydroxybenzoate-6-hydroxylase with cibacron blue
 
Creator SUMATHI, S
DASGUPTA, D
 
Subject dinucleotide fold
sepharose chromatography
lactate-dehydrogenase
klebsiella-pneumoniae
glutamine-synthetase
dextran-sepharose
purification
proteins
binding
6-hydroxylase
cibacron blue
circular dichroism
competitive
flavin
fluorescence
3-hydroxybenzoate
inhibition
uncompetitive
monooxygenase
3-hydroxybenzoate-6-hydroxylase
 
Description Cibacron blue is a potent inhibitor of 3-HBA-6-hydroxylase at a concentration < 1 mu M. Kinetic analyses revealed that at a concentration below 0.5 mu M the dye behaves as an uncompetitive inhibitor with respect to 3-HBA and competes with NADH for the same site on the enzyme. The alteration of the near-UV CD spectrum and quenching of the emission fluorescence of the enzyme by cibacron blue indicates a significant alteration in the environment of aromatic amino acid residues due to a stacking interaction and subtle conformatiodnal changes in the enzyme. The concentration-dependent quenching of the intrinsic fluorescence of the enzyme by cibacron blue was employed to determine the binding parameters such as association constant (K-a) and stoichiometry (r) for the enzyme-dye complex.
 
Publisher TAYLOR & FRANCIS LTD
 
Date 2011-08-31T00:51:24Z
2011-12-26T12:59:15Z
2011-12-27T05:50:21Z
2011-08-31T00:51:24Z
2011-12-26T12:59:15Z
2011-12-27T05:50:21Z
2006
 
Type Article
 
Identifier JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 21(1), 47-53
1475-6366
http://dx.doi.org/10.1080/02781070500323047
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12523
http://hdl.handle.net/10054/12523
 
Language en