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Multi-objective optimization in Aspergillus niger fermentation for selective product enhancement

DSpace at IIT Bombay

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Title Multi-objective optimization in Aspergillus niger fermentation for selective product enhancement
 
Creator MANDAL, CHAITALI
GUDI, RD
SURAISHKUMAR, GK
 
Subject fermentation
enzymes
nitrogen
oxygen
 
Description A multi-objective optimization formulation that reflects the multi-substrate optimization in a multi-product fermentation is proposed in this work. This formulation includes the application of ε-constraint to generate the trade-off solution for the enhancement of one selective product in a multi-product fermentation, with simultaneous minimization of the other product within a threshold limit. The formulation has been applied to the fed-batch fermentation of Aspergillus niger that produces a number of enzymes during the course of fermentation, and of these, catalase and protease enzyme expression have been chosen as the enzymes of interest. Also, this proposed formulation has been applied in the environment of three control variables, i.e. the feed rates of sucrose, nitrogen source and oxygen and a set of trade-off solutions have been generated to develop the pareto-optimal curve. We have developed and experimentally evaluated the optimal control profiles for multiple substrate feed additions in the fed-batch fermentation of A. niger to maximize catalase expression along with protease expression within a threshold limit and vice versa. An increase of about 70% final catalase and 31% final protease compared to conventional fed-batch cultivation were obtained. Novel methods of oxygen supply through liquid-phase H2O2 addition have been used with a view to overcome limitations of aeration due to high gas–liquid transport resistance. The multi-objective optimization problem involved linearly appearing control variables and the decision space is constrained by state and end point constraints. The proposed multi-objective optimization is solved by differential evolution algorithm, a relatively superior population-based stochastic optimization strategy.
 
Publisher Springer
 
Date 2009-11-26T04:40:51Z
2011-11-25T15:51:43Z
2011-12-26T13:04:50Z
2011-12-27T05:50:51Z
2009-11-26T04:40:51Z
2011-11-25T15:51:43Z
2011-12-26T13:04:50Z
2011-12-27T05:50:51Z
2005
 
Type Article
 
Identifier Bioprocess and Biosystems Engineering 28(3), 149-164
1615-7605
http://dx.doi.org/10.1007/s00449-005-0021-4
http://hdl.handle.net/10054/1718
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1718
 
Language en