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Labeling TiO(2) Nanoparticles with Dyes for Optical Fluorescence Microscopy and Determination of TiO(2)-DNA Nanoconjugate Stability

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Title Labeling TiO(2) Nanoparticles with Dyes for Optical Fluorescence Microscopy and Determination of TiO(2)-DNA Nanoconjugate Stability
 
Creator THURN, KT
PAUNESKU, T
WU, AG
BROWN, EMB
LAI, B
VOGT, S
MASER, J
ASLAM, M
DRAVID, V
BERGAN, R
WOLOSCHAK, GE
 
Subject titanium-dioxide nanoparticles
alizarin red-s
in-vitro
antisense oligonucleotides
intracellular trafficking
charge separation
binding proteins
mammalian-cells
ultrafine tio2
particles
dna
x-ray fluorescence microscopy
nanoconjugates
nanoparticles
tio(2)
 
Description Visualization of nanoparticles without intrinsic optical fluorescence properties is a significant problem when performing intracellular studies. Such is the case with titanium dioxide (TiO(2)) nanoparticles. These nanoparticles, when electronically linked to single-stranded DNA oligonucleotides, have been proposed to be used both as gene knockout devices and as possible tumor imaging agents. By interacting with complementary target sequences in living cells, these photoinducible TiO(2)-DNA nanoconjugates have the potential to cleave intracellular genomic DNA in a sequence specific and inducible manner. The nanoconjugates also become detectable by magnetic resonance imaging with the addition of gadolinium Gd(III) contrast agents. Herein two approaches for labeling TiO(2) nanoparticles and TiO(2)-DNA nanoconjugates with optically fluorescent agents are described. This permits direct quantification of fluorescently labeled TiO(2) nanoparticle uptake in a large population of living cells (>10(4) cells). X-ray fluorescence microscopy (XFM) is combined with fluorescent microscopy to determine the relative intracellular stability of the nanoconjugates and used to quantify intracellular nanoparticles. Imaging the DNA component of the TiO(2)-DNA nanoconjugate by fluorescent confocal microscopy within the same cell shows an overlap with the titanium signal as mapped by XFM. This strongly implies the intracellular integrity of the TiO(2)-DNA nanoconjugates in malignant cells.
 
Publisher WILEY-V C H VERLAG GMBH
 
Date 2011-09-01T18:09:08Z
2011-12-26T12:59:44Z
2011-12-27T05:52:32Z
2011-09-01T18:09:08Z
2011-12-26T12:59:44Z
2011-12-27T05:52:32Z
2009
 
Type Article
 
Identifier SMALL, 5(11), 1318-1325
1613-6810
http://dx.doi.org/10.1002/smll.200801458
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12835
http://hdl.handle.net/10054/12835
 
Language en