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A small-angle X-ray scattering study of the structure of lysozyme–sodium dodecyl sulfate complexes

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Title A small-angle X-ray scattering study of the structure of lysozyme–sodium dodecyl sulfate complexes
 
Creator NARAYANAN, JANAKY
ABDUL RASHEED, AS
BELLARE, JAYESH
 
Subject enzymes
paraffin waxes
sodium sulfate
x ray scattering
 
Description The structure of lysozyme–sodium dodecyl sulfate (SDS) complexes in solution is studied using small-angle X-ray scattering (SAXS). The SAXS data cannot be explained by the necklace and bead model for unfolded polypeptide chain interspersed with surfactant micelles. For the protein and surfactant concentrations used in the study, there is only marginal growth of SDS micelles as they complex with the protein. Being a small and rather rigid protein, lysozyme can penetrate the micellar core which is occupied by flexible and disordered paraffin chains and also the shell occupied by the hydrated head groups. A partially embedded swollen micellar model seems appropriate and describes well the scattering data. The SAXS intensity profiles are analyzed by considering the change in the electron scattering length density of the micellar core and shell due to complexation with protein and treating the intermicellar interaction using rescaled mean spherical approximation (RMSA) for charged spheres.
 
Publisher Elsevier
 
Date 2009-02-14T07:22:26Z
2011-11-25T16:48:23Z
2011-12-26T13:05:49Z
2011-12-27T05:53:43Z
2009-02-14T07:22:26Z
2011-11-25T16:48:23Z
2011-12-26T13:05:49Z
2011-12-27T05:53:43Z
2008
 
Type Article
 
Identifier Journal of Colloid and Interface Science 328(1), 67-72
0021-9797
10.1016/j.jcis.2008.09.012
http://hdl.handle.net/10054/636
http://dspace.library.iitb.ac.in/xmlui/handle/10054/636
 
Language en