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Autoregulation of regulatory proteins is key for dynamic operation of GAL switch in Saccharomyces cerevisiae

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Title Autoregulation of regulatory proteins is key for dynamic operation of GAL switch in Saccharomyces cerevisiae
 
Creator RUHELA, ANURAG
VERMA, MALKHEY
EDWARDS, JEREMY S
BHAT, PJ
BHARTIYA, SHARAD
VENKATESH, KV
 
Subject escherichia coli
dynamics
metabolites
 
Description Autoregulation and nucleocytoplasmic shuttling play important roles in the operation of the GAL regulatory system. However, the significance of these mechanisms in the overall operation of the switch is unclear. In this work, we develop a dynamic model for the GAL system and further validate the same using steady-state and dynamic experimental expression data. Next, the model is used to delineate the relevance of shuttling and autoregulation in response to inducing, repressing, and non-inducing–non-repressing media. The analysis indicates that autoregulation of the repressor, Gal80p, is key in obtaining three distinct steady states in response to the three media. In particular, the analysis rationalizes the intuitively paradoxical observation that the concentration of repressor, Gal80p, actually increases in response to an increase in the inducer concentration. On the other hand, although nucleocytoplasmic shuttling does not affect the dynamics of the system, it plays a dominant role in obtaining a sensitive response to galactose. The dynamic model was also used to obtain insights on the preculturing effect on the system behavior.
 
Publisher Elsevier
 
Date 2009-02-18T09:13:07Z
2011-11-25T17:13:57Z
2011-12-26T13:05:53Z
2011-12-27T05:53:47Z
2009-02-18T09:13:07Z
2011-11-25T17:13:57Z
2011-12-26T13:05:53Z
2011-12-27T05:53:47Z
2004
 
Type Article
 
Identifier FEBS Letters 576(1-2), 119-126
0014-5793
http://dx.doi.org/10.1016/j.febslet.2004.09.001
http://hdl.handle.net/10054/704
http://dspace.library.iitb.ac.in/xmlui/handle/10054/704
 
Language en