A double catgrip mixed L and D mini protein only 20 residues long
DSpace at IIT Bombay
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Title |
A double catgrip mixed L and D mini protein only 20 residues long
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Creator |
RANA, SOUMENDRA
KUNDU, BIJOY DURANI, SUSHEEL |
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Subject |
nuclear magnetic resonance
molecular dynamics circular dichroism synthesis |
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Description |
Stereochemistry limits but also defines proteins, as conformational constructs stereospecific for poly-L structure. Employed as a variable in sequence, stereochemistry could make proteins customizable in the letters of L and D amino acid alphabet. In proof of concept, we previously demonstrated stereochemical reengineering of canonical β-hairpins as bracelet and boat shaped molecules. Illustrating the prospect for functional design, a 20-residue four-stranded mini-β protein is now customized stereochemically as a canoe shaped molecule. A conformational construct of four side by side hydrogen-bonded strands in alternately Lβ, Dβ conformation, joined via Type-II/II′ β-turns, is planned to be preponderantly apolar in β-sheet favoring residues, interspersing two ion pairs, and suitably L and D in sequence. Synthesis followed by MD, NMR, CD, and MALDI-MS studies established the molecule as a canoe shaped fold in water, demonstrable in affinity of alkali and alkaline-earth metal ions as expected given its catgrip like elements. Another success in accomplishing a synthetic miniprotein complex in stereochemistry and stereospecific in conformation, exceptionally small yet functional in metal ion affinity, affirms the value in combined L and D alphabet in programming molecular shapes and functions stereochemically.
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Publisher |
Elsevier
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Date |
2009-02-21T11:28:22Z
2011-11-25T17:19:58Z 2011-12-26T13:06:10Z 2011-12-27T05:53:59Z 2009-02-21T11:28:22Z 2011-11-25T17:19:58Z 2011-12-26T13:06:10Z 2011-12-27T05:53:59Z 2007 |
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Type |
Article
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Identifier |
Bioorganic & Medicinal Chemistry 15(11), 3874-3882
0968-0896 http://dx.doi.org/10.1016/j.bmc.2007.03.030 http://hdl.handle.net/10054/731 http://dspace.library.iitb.ac.in/xmlui/handle/10054/731 |
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Language |
en
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