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A double catgrip mixed L and D mini protein only 20 residues long

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Title A double catgrip mixed L and D mini protein only 20 residues long
 
Creator RANA, SOUMENDRA
KUNDU, BIJOY
DURANI, SUSHEEL
 
Subject nuclear magnetic resonance
molecular dynamics
circular dichroism
synthesis
 
Description Stereochemistry limits but also defines proteins, as conformational constructs stereospecific for poly-L structure. Employed as a variable in sequence, stereochemistry could make proteins customizable in the letters of L and D amino acid alphabet. In proof of concept, we previously demonstrated stereochemical reengineering of canonical β-hairpins as bracelet and boat shaped molecules. Illustrating the prospect for functional design, a 20-residue four-stranded mini-β protein is now customized stereochemically as a canoe shaped molecule. A conformational construct of four side by side hydrogen-bonded strands in alternately Lβ, Dβ conformation, joined via Type-II/II′ β-turns, is planned to be preponderantly apolar in β-sheet favoring residues, interspersing two ion pairs, and suitably L and D in sequence. Synthesis followed by MD, NMR, CD, and MALDI-MS studies established the molecule as a canoe shaped fold in water, demonstrable in affinity of alkali and alkaline-earth metal ions as expected given its catgrip like elements. Another success in accomplishing a synthetic miniprotein complex in stereochemistry and stereospecific in conformation, exceptionally small yet functional in metal ion affinity, affirms the value in combined L and D alphabet in programming molecular shapes and functions stereochemically.
 
Publisher Elsevier
 
Date 2009-02-21T11:28:22Z
2011-11-25T17:19:58Z
2011-12-26T13:06:10Z
2011-12-27T05:53:59Z
2009-02-21T11:28:22Z
2011-11-25T17:19:58Z
2011-12-26T13:06:10Z
2011-12-27T05:53:59Z
2007
 
Type Article
 
Identifier Bioorganic & Medicinal Chemistry 15(11), 3874-3882
0968-0896
http://dx.doi.org/10.1016/j.bmc.2007.03.030
http://hdl.handle.net/10054/731
http://dspace.library.iitb.ac.in/xmlui/handle/10054/731
 
Language en