A differential scanning calorimetric study on the irreversible thermal unfolding of concanavalin A
DSpace at IIT Bombay
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Title |
A differential scanning calorimetric study on the irreversible thermal unfolding of concanavalin A
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Creator |
BANERJEE, TUHINA
NAND KISHORE |
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Subject |
calorimetry
concanavalin a irreversible thermal denaturation enthalpy activation energy |
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Description |
A differential scanning calorimetry study on the thermal denaturation of concanavalin A at pH 5.2 where it exists in the dimeric form was carried out. The calorimetric transitions were observed to be irreversible and the transition temperature of the protein increased with increasing scan rate, indicating that the thermal denaturation process is under kinetic control. The thermal unfolding, and its scan rate dependence could be explained according to the kinetic scheme N2 →k 2U with k as first-order kinetic constant whose change with temperature is given by the Arrhenius equation. Using this model, rate constant as a function of temperature and activation energy of the process have been calculated. The average activation energy of the kinetic process using different approaches is 129±10 kJ mol−1. The differential scanning calorimetric results on transition temperatures and calorimetric enthalpies supported by intrinsic fluorescence indicate that the irreversibility in the calorimetric transitions of concanavalin A includes a combination of post-transition aggregation, chain separation and loss of cofactor.
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Publisher |
Elsevier
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Date |
2009-03-02T09:30:51Z
2011-11-25T18:22:37Z 2011-12-26T13:06:33Z 2011-12-27T05:54:34Z 2009-03-02T09:30:51Z 2011-11-25T18:22:37Z 2011-12-26T13:06:33Z 2011-12-27T05:54:34Z 2004 |
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Type |
Article
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Identifier |
Thermochimica Acta 411(2), 195-201
0040-6031 http://dx.doi.org/10.1016/j.tca.2003.08.012 http://hdl.handle.net/10054/843 http://dspace.library.iitb.ac.in/xmlui/handle/10054/843 |
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Language |
en
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