Record Details

Interaction of 2,2,2-trifluoroethanol with proteins: calorimetric, densimetric and surface tension approach

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Interaction of 2,2,2-trifluoroethanol with proteins: calorimetric, densimetric and surface tension approach
 
Creator KUNDU, AGNITA
NAND KISHORE
 
Subject lysozyme
differential scanning calorimetry
partial molar heat capacity
partial molar volume
preferential interaction parameters
 
Description The thermal denaturation of hen egg-white lysozyme was studied in the presence of 2,2,2-trifluoroethanol (TFE) at various pH values using micro differential scanning calorimetry. Quantitative thermodynamic parameters accompanying the thermal transitions were evaluated. It is observed that thermal unfolding of lysozyme in the presence of TFE upto a concentration of 4.0 mol dm−3 follows a two-state denaturation mechanism as indicated by the equality of van't Hoff and calorimetric enthalpies. The finer details of interaction were studied by measuring the partial molar volume of some constituent amino acids and glycine peptides from water to aqueous TFE at 298.15 K. The physico-chemical properties of aqueous TFE: apparent molar heat capacities, apparent molar volumes and surface tension were measured to understand the intrinsic properties of the cosolvent as well. From the correlation among the thermal unfolding data on lysozyme in aqueous TFE, calculated preferential interaction parameters, physico chemical properties of aqueous TFE and partial molar volumes of transfer, it is concluded that both solvent mediated effect and direct interaction constitute the mechanism of TFE–protein interactions.
 
Publisher Elsevier
 
Date 2009-02-27T11:10:33Z
2011-11-25T18:10:06Z
2011-12-26T13:06:36Z
2011-12-27T05:54:36Z
2009-02-27T11:10:33Z
2011-11-25T18:10:06Z
2011-12-26T13:06:36Z
2011-12-27T05:54:36Z
2004
 
Type Article
 
Identifier Biophysical Chemistry 109(3), 427-442
0301-4622
http://dx.doi.org/10.1016/j.bpc.2003.12.009
http://hdl.handle.net/10054/818
http://dspace.library.iitb.ac.in/xmlui/handle/10054/818
 
Language en