4-Chlorobutanol induces unusual reversible and irreversible thermal unfolding of ribonuclease A: thermodynamic, kinetic, and conformational characterization
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Title |
4-Chlorobutanol induces unusual reversible and irreversible thermal unfolding of ribonuclease A: thermodynamic, kinetic, and conformational characterization
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Creator |
MEHTA, RANJANA
KUNDU, AGNITA NAND KISHORE |
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Subject |
denaturation
enthalpy differential scanning calorimetry activation energy |
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Description |
The thermal denaturation of ribonuclease A has been studied by differential scanning calorimetry in the presence of 4-chlorobutan-1-ol. The thermal transitions were observed to be reversible at pH 5.5 in the presence of low concentration (up to 50 mM) of the alcohol, irreversible in the intermediate (50 mM
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Publisher |
Elsevier
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Date |
2009-02-27T11:09:50Z
2011-11-25T18:08:05Z 2011-12-26T13:06:40Z 2011-12-27T05:54:41Z 2009-02-27T11:09:50Z 2011-11-25T18:08:05Z 2011-12-26T13:06:40Z 2011-12-27T05:54:41Z 2004 |
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Type |
Article
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Identifier |
International Journal of Biological Macromolecules 34(1-2), 13-20
0141-8130 http://dx.doi.org/10.1016/j.ijbiomac.2003.11.001 http://hdl.handle.net/10054/814 http://dspace.library.iitb.ac.in/xmlui/handle/10054/814 |
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Language |
en
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