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4-Chlorobutanol induces unusual reversible and irreversible thermal unfolding of ribonuclease A: thermodynamic, kinetic, and conformational characterization

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Title 4-Chlorobutanol induces unusual reversible and irreversible thermal unfolding of ribonuclease A: thermodynamic, kinetic, and conformational characterization
 
Creator MEHTA, RANJANA
KUNDU, AGNITA
NAND KISHORE
 
Subject denaturation
enthalpy
differential scanning calorimetry
activation energy
 
Description The thermal denaturation of ribonuclease A has been studied by differential scanning calorimetry in the presence of 4-chlorobutan-1-ol. The thermal transitions were observed to be reversible at pH 5.5 in the presence of low concentration (up to 50 mM) of the alcohol, irreversible in the intermediate (50 mM
 
Publisher Elsevier
 
Date 2009-02-27T11:09:50Z
2011-11-25T18:08:05Z
2011-12-26T13:06:40Z
2011-12-27T05:54:41Z
2009-02-27T11:09:50Z
2011-11-25T18:08:05Z
2011-12-26T13:06:40Z
2011-12-27T05:54:41Z
2004
 
Type Article
 
Identifier International Journal of Biological Macromolecules 34(1-2), 13-20
0141-8130
http://dx.doi.org/10.1016/j.ijbiomac.2003.11.001
http://hdl.handle.net/10054/814
http://dspace.library.iitb.ac.in/xmlui/handle/10054/814
 
Language en