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An integrated mechanistic model for transcription-coupled nucleotide excision repair

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Title An integrated mechanistic model for transcription-coupled nucleotide excision repair
 
Creator PATEL, SHWETAL
VENKATESH, KV
EDWARDS, JEREMY S
 
Subject cockayne syndrome
in silico biology
hypothesis
uvrabcd
 
Description Preferential repair of the transcribed strand of active genes is usually attributed to a coupling protein that dislodges RNA polymerase stalled at a damage site and recruits repair enzymes. Experimental observations of the effect of transcription on preferential repair in Escherichia coli are contradictory and inexplicable by this model. In this study, it is argued that the multiple conformations displayed by a stalled RNA polymerase result in two sub-pathways for repair: Mfd coupled and direct. Together with the fact that RNA polymerase recruits the repair enzymes in a promoter dependent manner, an integrated mechanistic model is proposed that is capable of explaining the effect of transcription on preferential repair reported in literature. The quantitative behavior of the model is illustrated by describing the various reactions using a biochemical network. The implications of the model on the mechanism for transcription-coupled repair in higher organisms are briefly discussed.
 
Publisher Elsevier
 
Date 2009-03-09T07:42:40Z
2011-11-25T18:47:41Z
2011-12-26T13:06:58Z
2011-12-27T05:54:57Z
2009-03-09T07:42:40Z
2011-11-25T18:47:41Z
2011-12-26T13:06:58Z
2011-12-27T05:54:57Z
2004
 
Type Article
 
Identifier DNA Repair 3(3), 343-348
1568-7864
http://dx.doi.org/10.1016/j.dnarep.2003.11.010
http://hdl.handle.net/10054/898
http://dspace.library.iitb.ac.in/xmlui/handle/10054/898
 
Language en