Effect of preculturing conditions on growth of Lactobacillus rhamnosus on medium containing glucose and citrate
DSpace at IIT Bombay
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Title |
Effect of preculturing conditions on growth of Lactobacillus rhamnosus on medium containing glucose and citrate
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Creator |
JYOTI, BD
SURESH, AK VENKATESH, KV |
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Subject |
cell
enzymes glucose growth kinetics |
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Description |
Lactobacillus rhamnosus can metabolize citrate through a citrate inducible transport system. The growth curves of L. rhamnosus on medium containing glucose and citrate was found to be highly dependent on preculturing conditions. It exhibited diauxic growth when precultured on glucose, but demonstrated simultaneous consumption when cultured on citrate. The maximum specific growth rate for cells growing on glucose+citrate was 0.38 h−1, which was higher than the growth rate on individual substrates (0.28 h−1). Simultaneous consumption also yielded higher net flavour compounds, diacetyl and acetoin. Flux analysis indicated that L. rhamnosus requires oxygen for balancing excess NADH through NADH oxidase. The flux analysis provided insights into the metabolic network of L. rhamnosus.
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Publisher |
Elsevier
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Date |
2009-03-09T07:40:37Z
2011-11-25T18:43:11Z 2011-12-26T13:07:02Z 2011-12-27T05:55:01Z 2009-03-09T07:40:37Z 2011-11-25T18:43:11Z 2011-12-26T13:07:02Z 2011-12-27T05:55:01Z 2004 |
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Type |
Article
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Identifier |
Microbiological Research 159(1), 35-42
0944-5013 http://dx.doi.org/10.1016/j.micres.2004.01.008 http://hdl.handle.net/10054/889 http://dspace.library.iitb.ac.in/xmlui/handle/10054/889 |
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Language |
en
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