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Effect of preculturing conditions on growth of Lactobacillus rhamnosus on medium containing glucose and citrate

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Title Effect of preculturing conditions on growth of Lactobacillus rhamnosus on medium containing glucose and citrate
 
Creator JYOTI, BD
SURESH, AK
VENKATESH, KV
 
Subject cell
enzymes
glucose
growth kinetics
 
Description Lactobacillus rhamnosus can metabolize citrate through a citrate inducible transport system. The growth curves of L. rhamnosus on medium containing glucose and citrate was found to be highly dependent on preculturing conditions. It exhibited diauxic growth when precultured on glucose, but demonstrated simultaneous consumption when cultured on citrate. The maximum specific growth rate for cells growing on glucose+citrate was 0.38 h−1, which was higher than the growth rate on individual substrates (0.28 h−1). Simultaneous consumption also yielded higher net flavour compounds, diacetyl and acetoin. Flux analysis indicated that L. rhamnosus requires oxygen for balancing excess NADH through NADH oxidase. The flux analysis provided insights into the metabolic network of L. rhamnosus.
 
Publisher Elsevier
 
Date 2009-03-09T07:40:37Z
2011-11-25T18:43:11Z
2011-12-26T13:07:02Z
2011-12-27T05:55:01Z
2009-03-09T07:40:37Z
2011-11-25T18:43:11Z
2011-12-26T13:07:02Z
2011-12-27T05:55:01Z
2004
 
Type Article
 
Identifier Microbiological Research 159(1), 35-42
0944-5013
http://dx.doi.org/10.1016/j.micres.2004.01.008
http://hdl.handle.net/10054/889
http://dspace.library.iitb.ac.in/xmlui/handle/10054/889
 
Language en