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Increase in enzyme productivity by induced oxidative stress in Bacillus subtilis cultures and analysis of its mechanism using microarray data

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Title Increase in enzyme productivity by induced oxidative stress in Bacillus subtilis cultures and analysis of its mechanism using microarray data
 
Creator MISHRA, SURABHI
NORONHA, SANTOSH
SURAISHKUMAR, GK
 
Subject enzyme kinetics
growth kinetics
data reduction
organic acids
 
Description Treatment of microbial cultures with chemical agents such as hydrogen peroxide (H2O2) and hypochlorous acid (HOCl) induces production of reactive oxygen species (ROS) in culture. These ROS inducing (treatment) agents increased the growth rate as well as the maximum specific levels of extracellular enzymes such as α-amylase and protease in Bacillus subtilis cultures. Treatment with hypochlorous acid increased maximum specific α-amylase level by 2.2-fold and maximum specific protease level by 2.6-fold, respectively. Similarly, treatment with H2O2 increased specific α-amylase and specific protease level by 1.5- and 1.9-fold, respectively. Increases in specific enzyme levels were correlated with levels of specific intracellular ROS in cultures. The mechanism of increase in enzyme productivity under induced oxidative stress was also traced at the genetic level through analysis of available microarray data. The microarray data showed an induced level of signal peptidase gene (sipT), which is the most important secretory apparatus component, and suggested that increased efficiency of secretory apparatus as a result of treatments with ROS inducing agents also leads to increased productivity of α-amylase.
 
Publisher Elsevier
 
Date 2009-03-17T10:04:15Z
2011-11-25T19:12:15Z
2011-12-26T13:07:31Z
2011-12-27T05:55:33Z
2009-03-17T10:04:15Z
2011-11-25T19:12:15Z
2011-12-26T13:07:31Z
2011-12-27T05:55:33Z
2005
 
Type Article
 
Identifier Process Biochemistry 40(5), 1863-1870
1359-5113
http://dx.doi.org/10.1016/j.procbio.2004.06.055
http://hdl.handle.net/10054/950
http://dspace.library.iitb.ac.in/xmlui/handle/10054/950
 
Language en