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Phenotypic characterization of Corynebacterium glutamicum under osmotic stress conditions using elementary mode analysis

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Title Phenotypic characterization of Corynebacterium glutamicum under osmotic stress conditions using elementary mode analysis
 
Creator RAJVANSHI, M
VENKATESH, KV
 
Subject PATHWAY ANALYSIS
BREVIBACTERIUM-LACTOFERMENTUM
HYPEROSMOTIC STRESS
2-COMPONENT SYSTEM
ACCUMULATION
TREHALOSE
BACTERIA
GROWTH
VOLUME
MTRB
Corynebacterium glutamicum
Osmotic stress
Elementary mode analysis
Nodal analysis
Flux analysis
 
Description Corynebacterium glutamicum, a soil bacterium, is used to produce amino acids such as lysine and glutamate. C. glutamicum is often exposed to osmolality changes in its medium, and the bacterium has therefore evolved several adaptive response mechanisms to overcome them. In this study we quantify the metabolic response of C. glutamicum under osmotic stress using elementary mode analysis (EMA). Further, we obtain the optimal phenotypic space for the synthesis of lysine and formation of biomass. The analysis demonstrated that with increasing osmotic stress, the flux towards trehalose formation and energy-generating pathways increased, while the flux of anabolic reactions diminished. Nodal analysis indicated that glucose-6-phosphate, phosphoenol pyruvate, and pyruvate nodes were capable of adapting to osmotic stress, whereas the oxaloacetic acid node was relatively unresponsive. Fewer elementary modes were active under stress indicating the rigid behavior of the metabolism in response to high osmolality. Optimal phenotypic space analysis revealed that under normal conditions the organism optimized growth during the initial log phase and lysine and trehalose formation during the stationary phase. However, under osmotic stress, the analysis demonstrated that the organism operates under suboptimal conditions for growth, and lysine and trehalose formation.
 
Publisher SPRINGER HEIDELBERG
 
Date 2012-06-26T05:21:23Z
2012-06-26T05:21:23Z
2011
 
Type Article
 
Identifier JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,38(9)1345-1357
1367-5435
http://dx.doi.org/10.1007/s10295-010-0918-z
http://dspace.library.iitb.ac.in/jspui/handle/100/13955
 
Language English