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Multifunctional alginate microspheres for biosensing, drug delivery and magnetic resonance imaging

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Title Multifunctional alginate microspheres for biosensing, drug delivery and magnetic resonance imaging
 
Creator JOSHI, A
SOLANKI, S
CHAUDHARI, R
BAHADUR, D
ASLAM, M
SRIVASTAVA, R
 
Subject IRON-OXIDE NANOPARTICLES
IN-VIVO EVALUATION
RECOMBINANT CELLS
GLUCOSE-OXIDASE
ENCAPSULATION
PARTICLES
FE3O4
ASSAY
DEXAMETHASONE
MEMBRANE
Magnetic nanoparticles
Alginate microspheres
Biosensor
Drug delivery
MRI
 
Description This research aims to develop and investigate a multifunctional implantable system capable of biosensing, drug delivery and magnetic resonance imaging (MRI) for continuous monitoring, controlled anti-inflammatory drug delivery and imaging, respectively. A glucose biosensor, diclofenac sodium (Diclo) and magnetic nanoparticles (MNP) were used as the biosensor component, anti-inflammatory agent and MRI contrast agent, respectively. MNP were synthesized by the co-precipitation technique and loaded with the sensor and drug components into alginate microspheres using a commercial droplet generator. The multifunctional system was then characterized using optical microscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, vibrating sample magnetometry (VSM) and MRI. The MNP were found to be in the size range of 5-15 nm. The final system, comprising the biosensor, drug and MNP loaded inside alginate microspheres, was found to be in the size range of 10-60 mu m. Biosensing studies indicated an excellent glucose response curve, with a regression coefficient of 0.974 (0-10 mM of glucose, response time: 4 min). In vitro Diclo release shows that MNP loading in alginate microspheres increases the burst release percentage by 11-12% in both 60 and 10 pm particles. However, the duration of release for 85% drug release decreases with MNP loading by 7 and 6 days for 39 the 60 and 10 pm particles, respectively. Super-paramagnetism was confirmed by VSM, with 2.09 and 1.368 emu g(-1), respectively, for the 60 and 10 pm particles, with no hysteresis. MRI showed significant contrast for both sizes. The particles showed an excellent biocompatibility (>80%) for all combinations of formulations. The system shows a great potential for biosensing with concurrent drug delivery and visualization for biomedical applications. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
 
Publisher ELSEVIER SCI LTD
 
Date 2012-06-26T06:08:36Z
2012-06-26T06:08:36Z
2011
 
Type Article
 
Identifier ACTA BIOMATERIALIA,7(11)3955-3963
1742-7061
http://dx.doi.org/10.1016/j.actbio.2011.06.053
http://dspace.library.iitb.ac.in/jspui/handle/100/14008
 
Language English