Multifunctional alginate microspheres for biosensing, drug delivery and magnetic resonance imaging
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Title |
Multifunctional alginate microspheres for biosensing, drug delivery and magnetic resonance imaging
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Creator |
JOSHI, A
SOLANKI, S CHAUDHARI, R BAHADUR, D ASLAM, M SRIVASTAVA, R |
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Subject |
IRON-OXIDE NANOPARTICLES
IN-VIVO EVALUATION RECOMBINANT CELLS GLUCOSE-OXIDASE ENCAPSULATION PARTICLES FE3O4 ASSAY DEXAMETHASONE MEMBRANE Magnetic nanoparticles Alginate microspheres Biosensor Drug delivery MRI |
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Description |
This research aims to develop and investigate a multifunctional implantable system capable of biosensing, drug delivery and magnetic resonance imaging (MRI) for continuous monitoring, controlled anti-inflammatory drug delivery and imaging, respectively. A glucose biosensor, diclofenac sodium (Diclo) and magnetic nanoparticles (MNP) were used as the biosensor component, anti-inflammatory agent and MRI contrast agent, respectively. MNP were synthesized by the co-precipitation technique and loaded with the sensor and drug components into alginate microspheres using a commercial droplet generator. The multifunctional system was then characterized using optical microscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, vibrating sample magnetometry (VSM) and MRI. The MNP were found to be in the size range of 5-15 nm. The final system, comprising the biosensor, drug and MNP loaded inside alginate microspheres, was found to be in the size range of 10-60 mu m. Biosensing studies indicated an excellent glucose response curve, with a regression coefficient of 0.974 (0-10 mM of glucose, response time: 4 min). In vitro Diclo release shows that MNP loading in alginate microspheres increases the burst release percentage by 11-12% in both 60 and 10 pm particles. However, the duration of release for 85% drug release decreases with MNP loading by 7 and 6 days for 39 the 60 and 10 pm particles, respectively. Super-paramagnetism was confirmed by VSM, with 2.09 and 1.368 emu g(-1), respectively, for the 60 and 10 pm particles, with no hysteresis. MRI showed significant contrast for both sizes. The particles showed an excellent biocompatibility (>80%) for all combinations of formulations. The system shows a great potential for biosensing with concurrent drug delivery and visualization for biomedical applications. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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Publisher |
ELSEVIER SCI LTD
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Date |
2012-06-26T06:08:36Z
2012-06-26T06:08:36Z 2011 |
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Type |
Article
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Identifier |
ACTA BIOMATERIALIA,7(11)3955-3963
1742-7061 http://dx.doi.org/10.1016/j.actbio.2011.06.053 http://dspace.library.iitb.ac.in/jspui/handle/100/14008 |
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Language |
English
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