Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280 nm wavelength
DSpace at IIT Bombay
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Title |
Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280 nm wavelength
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Creator |
BHARADWAJ, R
SAI, VVR THAKARE, K DHAWANGALE, A KUNDU, T TITUS, S VERMA, PK MUKHERJI, S |
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Subject |
FIELD ABSORPTION SENSOR
U-SHAPED PROBE GUIDE SENSOR SENSITIVITY BACTERIA O157-H7 CELLS Evanescent wave biosensor U-bent fiber optic probe E. coli 280 nm |
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Description |
A novel label-free technique for the detection of pathogens based on evanescent wave absorbance (EWA) changes at 280 nm from a U-bent optical fiber sensor is demonstrated. Bending a decladded fiber into a U-shaped structure enhances the penetration depth of evanescent waves and hence sensitivity of the probe. We show that the enhanced EWA response from such U-bent probes, caused by the inherent optical absorbance properties of bacterial cells or biomolecules specifically bound to the sensor surface, can be exploited for the detection of pathogens. A portable optical set-up with a UV light emitting diode, a spectrometer and U-bent fiber optic probe of 200 core diameter, 0.75 mm bend radius and effective probe length of 1 cm demonstrated an ability to detect less than 1000 cfu/ml. (C) 2011 Published by Elsevier B.V.
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Publisher |
ELSEVIER ADVANCED TECHNOLOGY
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Date |
2012-06-26T06:23:48Z
2012-06-26T06:23:48Z 2011 |
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Type |
Article
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Identifier |
BIOSENSORS & BIOELECTRONICS,26(7)3367-3370
0956-5663 http://dx.doi.org/10.1016/j.bios.2010.12.014 http://dspace.library.iitb.ac.in/jspui/handle/100/14028 |
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Language |
English
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