Effect of D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on surfactant monolayers
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Title |
Effect of D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on surfactant monolayers
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Creator |
SHAH, AR
BANERJEE, R |
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Subject |
RESPIRATORY-DISTRESS-SYNDROME
ACUTE LUNG INJURY PULMONARY SURFACTANT OLEIC-ACID N-ACETYLCYSTEINE FILM FORMATION VITAMIN-E IN-VITRO MODEL FLUORESCENCE D-alpha-Tocopheryl polyethylene glycol 1000 succinate Pulmonary surfactant Surface tension Liposomes Monolayer Capillary surfactometer |
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Description |
In the present study, the effects of an amphiphilic polymer, D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on model surfactant monolayers dipalmitoylphosphatidylcholine (DPPC), a binary mixture of DPPC with palmitoyloleoyl phosphatidylglycerol (DPPC-POPG) 9:1 (w/w) and binary mixture of DPPC and oleic acid (DPPC-OA) were evaluated. The ability of TPGS to act as an antioxidant adjuvant for pulmonary surfactants was also evaluated. Compression isotherms of surfactant monolayers at 37 degrees C in a Langmuir-Blodgett trough showed that DPPC and DPPC:TPGS mixed monolayers (1:0.25-1:1, w/w) exhibited low minimum surface tensions (MST) of 1-2 mN/m. Similarly [DPPC:POPG (9:1, w/w)]:TPGS mixed films of 1:0.25-1:1 weight ratios reached 1-2 mN/m MST. DPPC:POPG:TPGS liposomes adsorbed to surface tensions of 29-31 mN/m within 1 s. While monolayers of DPPC:OA (1:1, w/w) reached high MST of similar to 11 mN/m, DPPC:OA:TPGS (1:1:0.25, w/w) film reached near zero MST suggesting that low concentrations of TPGS reverses the effect of OA on DPPC monolayer. Capillary surfactometer studies showed DPPC:TPGS and [DPPC:POPG (9:1, w/w)]:TPGS liposomes maintained 84-95% airway patency. Fluorescence spectroscopy of Laurdan loaded DPPC:TPGS and DPPC:POPG:TPGS liposomes revealed no segregation of lipid domains in the lipid bilayer. Addition of TPGS to soybean liposome significantly reduced thiobarbituric acid reactive substance (TBARS) by 29-39% confirming its antioxidant nature. The results suggest a potential use of TPGS as an adjuvant to improve the surfactant activity as well as act as an antioxidant by scavenging free radicals. (C) 2011 Elsevier B.V. All rights reserved.
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Publisher |
ELSEVIER SCIENCE BV
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Date |
2012-06-26T06:29:50Z
2012-06-26T06:29:50Z 2011 |
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Type |
Article
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Identifier |
COLLOIDS AND SURFACES B-BIOINTERFACES,85(2)116-124
0927-7765 http://dx.doi.org/10.1016/j.colsurfb.2011.01.021 http://dspace.library.iitb.ac.in/jspui/handle/100/14040 |
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Language |
English
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