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Effect of D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on surfactant monolayers

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Title Effect of D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on surfactant monolayers
 
Creator SHAH, AR
BANERJEE, R
 
Subject RESPIRATORY-DISTRESS-SYNDROME
ACUTE LUNG INJURY
PULMONARY SURFACTANT
OLEIC-ACID
N-ACETYLCYSTEINE
FILM FORMATION
VITAMIN-E
IN-VITRO
MODEL
FLUORESCENCE
D-alpha-Tocopheryl polyethylene glycol 1000 succinate
Pulmonary surfactant
Surface tension
Liposomes
Monolayer
Capillary surfactometer
 
Description In the present study, the effects of an amphiphilic polymer, D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on model surfactant monolayers dipalmitoylphosphatidylcholine (DPPC), a binary mixture of DPPC with palmitoyloleoyl phosphatidylglycerol (DPPC-POPG) 9:1 (w/w) and binary mixture of DPPC and oleic acid (DPPC-OA) were evaluated. The ability of TPGS to act as an antioxidant adjuvant for pulmonary surfactants was also evaluated. Compression isotherms of surfactant monolayers at 37 degrees C in a Langmuir-Blodgett trough showed that DPPC and DPPC:TPGS mixed monolayers (1:0.25-1:1, w/w) exhibited low minimum surface tensions (MST) of 1-2 mN/m. Similarly [DPPC:POPG (9:1, w/w)]:TPGS mixed films of 1:0.25-1:1 weight ratios reached 1-2 mN/m MST. DPPC:POPG:TPGS liposomes adsorbed to surface tensions of 29-31 mN/m within 1 s. While monolayers of DPPC:OA (1:1, w/w) reached high MST of similar to 11 mN/m, DPPC:OA:TPGS (1:1:0.25, w/w) film reached near zero MST suggesting that low concentrations of TPGS reverses the effect of OA on DPPC monolayer. Capillary surfactometer studies showed DPPC:TPGS and [DPPC:POPG (9:1, w/w)]:TPGS liposomes maintained 84-95% airway patency. Fluorescence spectroscopy of Laurdan loaded DPPC:TPGS and DPPC:POPG:TPGS liposomes revealed no segregation of lipid domains in the lipid bilayer. Addition of TPGS to soybean liposome significantly reduced thiobarbituric acid reactive substance (TBARS) by 29-39% confirming its antioxidant nature. The results suggest a potential use of TPGS as an adjuvant to improve the surfactant activity as well as act as an antioxidant by scavenging free radicals. (C) 2011 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2012-06-26T06:29:50Z
2012-06-26T06:29:50Z
2011
 
Type Article
 
Identifier COLLOIDS AND SURFACES B-BIOINTERFACES,85(2)116-124
0927-7765
http://dx.doi.org/10.1016/j.colsurfb.2011.01.021
http://dspace.library.iitb.ac.in/jspui/handle/100/14040
 
Language English