Record Details

In vitro and in vivo evaluation of anti-inflammatory agents using nanoengineered alginate carriers: Towards localized implant inflammation suppression

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title In vitro and in vivo evaluation of anti-inflammatory agents using nanoengineered alginate carriers: Towards localized implant inflammation suppression
 
Creator JAYANT, RD
MCSHANE, MJ
SRIVASTAVA, R
 
Subject ENCAPSULATES SUBCUTANEOUS IMPLANTS
GLUCOSE SENSORS
PLGA MICROSPHERES
NANOFILM COATINGS
TISSUE-RESPONSE
DEXAMETHASONE
BIOSENSORS
RELEASE
HYDROGELS
POLYMERS
Alginate
Microspheres
Controlled release
Anti-inflammatory drugs
Layer-by-layer (LbL)
 
Description The aim of this research was to develop nanoengineered alginate microspheres for localized delivery of anti-inflammatory drugs (dexamethasone and diclofenac sodium) for implantable "Smart tattoo" glucose biosensor used for continuous glucose monitoring. The formulation was prepared and characterized for in vitro drug release from uncoated and polyelectrolyte-coated microparticles. Biocompatibility was then tested using L929 cell-line; pilot in vivo studies with Sprague-Dawley (SD) rat subjects were performed to test the suppression of inflammation and fibrosis associated with implantation and was analyzed using standard hematoxylin and eosin staining method. The drug-loaded microspheres were able to deliver the drug for 30 days at a controlled rate with zero-order kinetics. The layer-by-layer self-assembly technique was used to effectively limit the burst release of drug from the matrix. Cell culture studies prove that the material are not cytotoxic and showed acceptable >80% cell viability in all the tested samples. In vivo studies show that both drugs were successful in controlling the implant/tissue interface by suppressing inflammation at the implant site. It was clearly evident that the combined approach of drug loaded carriers along with implanted biosensor shows promise in improving sensor biocompatibility and functionality. Thus, suggesting potential application of alginate microspheres as "smart-tattoo" glucose sensors. (C) 2010 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2012-06-26T07:07:00Z
2012-06-26T07:07:00Z
2011
 
Type Article
 
Identifier INTERNATIONAL JOURNAL OF PHARMACEUTICS,403(1-2)268-275
0378-5173
http://dx.doi.org/10.1016/j.ijpharm.2010.10.035
http://dspace.library.iitb.ac.in/jspui/handle/100/14093
 
Language English