Utility of Aspergillus niger citrate synthase promoter for heterologous expression
DSpace at IIT Bombay
View Archive InfoField | Value | |
Title |
Utility of Aspergillus niger citrate synthase promoter for heterologous expression
|
|
Creator |
DAVE, K
PUNEKAR, NS |
|
Subject |
CITRIC-ACID FERMENTATION
DELETION ANALYSIS NIDULANS FUNGUS DEHYDROGENASE VERSATILE ORYZAE Citrate synthase Fungal promoter Gene constructs Aspergillus niger Protein expression |
|
Description |
Citrate synthase is a central player in the acidogenic metabolism of Aspergillus niger. The 5' upstream sequence (0.9 kb DNA) of citrate synthase gene (citA) from A. niger NCIM 565 was analyzed and its promoter function demonstrated through the heterologous expression of two proteins. The cloned citrate synthase promoter (PcitA) sequence was able to express bar coding sequence thereby conferring phosphinothricin resistance. This sequence was further analyzed by systematic deletions to define an effective but compact functional promoter. The PcitA driven egfp expression showed that PcitA was active in all differentiation cell-stages of A. niger. EGFP expression was highest on non-repressible carbon sources like acetate and glycerol. Mycelial EGFP levels increased during acidogenic growth suggesting that PcitA is functional throughout this cultivation. A. niger PcitA is the first Krebs cycle gene promoter used to express heterologous proteins in filamentous fungi. (C) 2011 Elsevier B.V. All rights reserved.
|
|
Publisher |
ELSEVIER SCIENCE BV
|
|
Date |
2012-06-26T07:10:31Z
2012-06-26T07:10:31Z 2011 |
|
Type |
Article
|
|
Identifier |
JOURNAL OF BIOTECHNOLOGY,155(2)173-177
0168-1656 http://dx.doi.org/10.1016/j.jbiotec.2011.06.012 http://dspace.library.iitb.ac.in/jspui/handle/100/14100 |
|
Language |
English
|
|