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Utility of Aspergillus niger citrate synthase promoter for heterologous expression

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Title Utility of Aspergillus niger citrate synthase promoter for heterologous expression
 
Creator DAVE, K
PUNEKAR, NS
 
Subject CITRIC-ACID FERMENTATION
DELETION ANALYSIS
NIDULANS
FUNGUS
DEHYDROGENASE
VERSATILE
ORYZAE
Citrate synthase
Fungal promoter
Gene constructs
Aspergillus niger
Protein expression
 
Description Citrate synthase is a central player in the acidogenic metabolism of Aspergillus niger. The 5' upstream sequence (0.9 kb DNA) of citrate synthase gene (citA) from A. niger NCIM 565 was analyzed and its promoter function demonstrated through the heterologous expression of two proteins. The cloned citrate synthase promoter (PcitA) sequence was able to express bar coding sequence thereby conferring phosphinothricin resistance. This sequence was further analyzed by systematic deletions to define an effective but compact functional promoter. The PcitA driven egfp expression showed that PcitA was active in all differentiation cell-stages of A. niger. EGFP expression was highest on non-repressible carbon sources like acetate and glycerol. Mycelial EGFP levels increased during acidogenic growth suggesting that PcitA is functional throughout this cultivation. A. niger PcitA is the first Krebs cycle gene promoter used to express heterologous proteins in filamentous fungi. (C) 2011 Elsevier B.V. All rights reserved.
 
Publisher ELSEVIER SCIENCE BV
 
Date 2012-06-26T07:10:31Z
2012-06-26T07:10:31Z
2011
 
Type Article
 
Identifier JOURNAL OF BIOTECHNOLOGY,155(2)173-177
0168-1656
http://dx.doi.org/10.1016/j.jbiotec.2011.06.012
http://dspace.library.iitb.ac.in/jspui/handle/100/14100
 
Language English