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Nucleosome positioning in a model of active chromatin remodeling enzymes

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Title Nucleosome positioning in a model of active chromatin remodeling enzymes
 
Creator PADINHATEERI, R
MARKO, JF
 
Subject XENOPUS EGG EXTRACTS
SEQUENCE PREFERENCES
DNA
YEAST
GENOME
COMPLEXES
DYNAMICS
FIBERS
ACF
ATP-dependent nucleosome removal
ATP-dependent nucleosome sliding
protein-DNA interactions
chromatin dynamics
 
Description Accounting for enzyme-mediated active sliding, disassembly, and sequence-dependent positioning of nucleosomes, we simulate nucleosome occupancy over cell-cycle-scale times using a stochastic kinetic model. We show that ATP-dependent active nucleosome sliding and nucleosome removal processes are essential to obtain in vivo-like nucleosome positioning. While active sliding leads to dense nucleosome filling, sliding events alone cannot ensure sequence-dependent nucleosome positioning: Active nucleosome removal is the crucial remodeling event that drives positioning. We also show that remodeling activity changes nucleosome dynamics from glassy to liquid-like, and that remodeling dramatically influences exposure dynamics of promoter regions.
 
Publisher NATL ACAD SCIENCES
 
Date 2012-06-26T09:52:55Z
2012-06-26T09:52:55Z
2011
 
Type Article
 
Identifier PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,108(19)7799-7803
0027-8424
http://dx.doi.org/10.1073/pnas.1015206108
http://dspace.library.iitb.ac.in/jspui/handle/100/14336
 
Language English