Record Details

Influence of cell spreading and contractility on stiffness measurements using AFM

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Influence of cell spreading and contractility on stiffness measurements using AFM
 
Creator VICHARE, S
INAMDAR, MM
SEN, S
 
Subject ATOMIC-FORCE MICROSCOPY
MECHANICAL-PROPERTIES
VISCOELASTIC PROPERTIES
ENDOTHELIAL-CELLS
ADHESION DYNAMICS
STEM-CELLS
SUBSTRATE
INDENTATION
ELASTICITY
NUCLEUS
 
Description Atomic Force Microscopy (AFM) is widely used for measuring mechanical properties of cells, and to understand how cells respond to their mechanical environments. A standard method for obtaining cell stiffness from experimental force-indentation curves is based on the simplified Hertz theory developed for studying the indentation of a semi-infinite elastic body by a spherical punch, assumptions that do not hold for biological cells. The modified Hertz theory developed by Dimitriadis et al., which takes the finite sample height into account, is widely used by experimentalists for greater accuracy. However, neither of these two models account for the finite lateral spread of the cells and cellular contractility. In this paper, we address the influence of cell geometry, cell pre-stress, and nuclear properties on cell stiffness measurements by modeling indentation of a cell of prescribed geometry with a spherical AFM probe using the finite element method. Using parametric studies, we develop scaling relationships between the effective stiffness probed by AFM and the bulk cell stiffness, taking cell and tip geometry into account. Taken together, our results demonstrate the need to take cell geometry into account while estimating the cell stiffness and provide simple expressions for doing so.
 
Publisher ROYAL SOC CHEMISTRY
 
Date 2014-10-14T12:58:59Z
2014-10-14T12:58:59Z
2012
 
Type Article
 
Identifier SOFT MATTER, 8(40)10464-10471
http://dx.doi.org/10.1039/c2sm26348c
http://dspace.library.iitb.ac.in/jspui/handle/100/14464
 
Language en