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Metabolic flexibility of D-ribose producer strain of Bacillus pumilus under environmental perturbations

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Title Metabolic flexibility of D-ribose producer strain of Bacillus pumilus under environmental perturbations
 
Creator SRIVASTAVA, RK
MAITI, SK
DAS, D
BAPAT, PM
BATTA, K
BHUSHAN, M
WANGIKAR, PP
 
Subject Flux analysis
Transketolase deficient
D-Ribose
Sensitivity
Reliability
CENTRAL CARBON METABOLISM
RIFAMYCIN-B FERMENTATION
OXYGEN-FREE RADICALS
ESCHERICHIA-COLI
FLUX ANALYSIS
SUBTILIS METABOLISM
GLUCOSE
RIBOFLAVIN
MODEL
METHYLGLYOXAL
 
Description The metabolic reaction rate vector is a bridge that links gene and protein expression alterations to the phenotypic endpoint. We present a simple approach for the estimation of flux distribution at key branch points in the metabolic network by using substrate uptake, metabolite secretion rate, and biomass growth rate for transketolase (tkt) deficient Bacillus pumilus ATCC 21951. We find that the glucose-6-phosphate (G6P) and pseudo catabolic/anabolic branch points are flexible in the d-ribose-producing tkt deficient strain of B. pumilus. The normalized flux through the pentose phosphate pathway (PPP) varied from 1.5 to 86 % under different growth conditions, thereby enabling substantial extracellular accumulation of d-ribose under certain conditions. Interestingly, the flux through PPP was affected by the extracellular phosphate concentration and dissolved oxygen concentration. This metabolic flexibility may have been the underlying reason for this strain being selected from thousands of others in a screening for d-ribose producers conducted in the 1970s.
 
Publisher SPRINGER HEIDELBERG
 
Date 2014-10-14T17:29:51Z
2014-10-14T17:29:51Z
2012
 
Type Article
 
Identifier JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 39(8)1227-1243
http://dx.doi.org/10.1007/s10295-012-1115-z
http://dspace.library.iitb.ac.in/jspui/handle/100/14571
 
Language en