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Metabolic regulation and chromosomal localization of carbaryl degradation pathway in Pseudomonas sp strains C4, C5 and C6

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Title Metabolic regulation and chromosomal localization of carbaryl degradation pathway in Pseudomonas sp strains C4, C5 and C6
 
Creator SINGH, R
TRIVEDI, VD
PHALE, PS
 
Subject Carbaryl degradation
Enzyme induction
Upper and lower pathways
Plasmid stability and curing
Chromosomal localization of genes
Salicylate 5-hydroxylase
PHTHALATE DEGRADATION
CRYPTIC PLASMIDS
PUTIDA CSV86
PURIFICATION
1-NAPHTHOL
GENES
SALICYLATE
ORGANIZATION
SEQUENCE
SOIL
 
Description Pseudomonas sp. strains C4, C5 and C6 degrade carbaryl (1-naphthyl N-methylcarbamate) via 1-naphthol, 1,2-dihydroxynaphthalene, salicylate and gentisate. Carbon source-dependent metabolic studies suggest that enzymes responsible for carbaryl degradation are probably organized into 'upper' (carbaryl to salicylate), 'middle' (salicylate to gentisate) and 'lower' (gentisate to TCA cycle) pathway. Carbaryl and 1-naphthol were found to induce all carbaryl pathway enzymes, while salicylate and gentisate induce middle and lower pathway enzymes. The strains were found to harbor plasmid(s), and carbaryl degradation property was found to be stable. Genes encoding enzymes of the degradative pathway such as 1-naphthol 2-hydroxylase, salicylaldehyde dehydrogenase, salicylate 5-hydroxylase and gentisate 1,2-dioxygenase were amplified from chromosomal DNA of these strains. The gene-specific PCR products were sequenced from strain C6, and phylogenetic tree was constructed. Southern hybridization and PCR analysis using gel eluted DNA as template supported the presence of pathway genes onto the chromosome and not on the plasmid(s).
 
Publisher SPRINGER
 
Date 2014-10-15T08:01:50Z
2014-10-15T08:01:50Z
2013
 
Type Article
 
Identifier ARCHIVES OF MICROBIOLOGY, 195(8)521-535
http://dx.doi.org/10.1007/s00203-013-0903-9
http://dspace.library.iitb.ac.in/jspui/handle/100/14631
 
Language en