Metabolic regulation and chromosomal localization of carbaryl degradation pathway in Pseudomonas sp strains C4, C5 and C6
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Title |
Metabolic regulation and chromosomal localization of carbaryl degradation pathway in Pseudomonas sp strains C4, C5 and C6
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Creator |
SINGH, R
TRIVEDI, VD PHALE, PS |
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Subject |
Carbaryl degradation
Enzyme induction Upper and lower pathways Plasmid stability and curing Chromosomal localization of genes Salicylate 5-hydroxylase PHTHALATE DEGRADATION CRYPTIC PLASMIDS PUTIDA CSV86 PURIFICATION 1-NAPHTHOL GENES SALICYLATE ORGANIZATION SEQUENCE SOIL |
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Description |
Pseudomonas sp. strains C4, C5 and C6 degrade carbaryl (1-naphthyl N-methylcarbamate) via 1-naphthol, 1,2-dihydroxynaphthalene, salicylate and gentisate. Carbon source-dependent metabolic studies suggest that enzymes responsible for carbaryl degradation are probably organized into 'upper' (carbaryl to salicylate), 'middle' (salicylate to gentisate) and 'lower' (gentisate to TCA cycle) pathway. Carbaryl and 1-naphthol were found to induce all carbaryl pathway enzymes, while salicylate and gentisate induce middle and lower pathway enzymes. The strains were found to harbor plasmid(s), and carbaryl degradation property was found to be stable. Genes encoding enzymes of the degradative pathway such as 1-naphthol 2-hydroxylase, salicylaldehyde dehydrogenase, salicylate 5-hydroxylase and gentisate 1,2-dioxygenase were amplified from chromosomal DNA of these strains. The gene-specific PCR products were sequenced from strain C6, and phylogenetic tree was constructed. Southern hybridization and PCR analysis using gel eluted DNA as template supported the presence of pathway genes onto the chromosome and not on the plasmid(s).
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Publisher |
SPRINGER
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Date |
2014-10-15T08:01:50Z
2014-10-15T08:01:50Z 2013 |
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Type |
Article
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Identifier |
ARCHIVES OF MICROBIOLOGY, 195(8)521-535
http://dx.doi.org/10.1007/s00203-013-0903-9 http://dspace.library.iitb.ac.in/jspui/handle/100/14631 |
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Language |
en
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