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Amyloid-Like Fibril Formation by Tachykinin Neuropeptides and Its Relevance to Amyloid beta-Protein Aggregation and Toxicity

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Title Amyloid-Like Fibril Formation by Tachykinin Neuropeptides and Its Relevance to Amyloid beta-Protein Aggregation and Toxicity
 
Creator SINGH, PK
MAJI, SK
 
Subject Neuropeptides
Amyloids
Functions
Fibrils
Peptide
X-RAY-DIFFRACTION
ALPHA-SYNUCLEIN
ALZHEIMERS-DISEASE
SECRETORY GRANULES
SUBSTANCE-P
IN-VITRO
PEPTIDES
SEQUENCE
GLYCOSAMINOGLYCANS
TRIFLUOROETHANOL
 
Description Protein aggregation and amyloid formation are associated with both pathological conditions in humans such as Alzheimer's disease and native functions such as peptide hormone storage in the pituitary secretory granules in mammals. Here, we studied amyloid fibrils formation by three neuropeptides namely physalaemin, kassinin and substance P of tachykinin family using biophysical techniques including circular dichroism, thioflavin T, congo red binding and microscopy. All these neuropeptides under study have significant sequence similarity with A beta(25-35) that is known to form neurotoxic amyloids. We found that all these peptides formed amyloid-like fibrils in vitro in the presence of heparin, and these amyloids were found to be nontoxic in neuronal cells. However, the extent of amyloid formation, structural transition, and morphology were different depending on the primary sequences of peptide. When A beta(25-35) and A beta 40 were incubated with each of these neuropeptides in 1: 1 ratio, a drastic increase in amyloid growths were observed compared to that of individual peptides suggesting that co-aggregation of A beta and these neuropeptides. The electron micrographs of these co-aggregates were dissimilar when compared with individual peptide fibrils further supporting the possible incorporation of these neuropeptides in A beta b amyloid fibrils. Further, the fibrils of these neuropeptides can seed the fibrils formation of A beta 40 and reduced the toxicity of preformed A beta fibrils. The present study of amyloid formation by tachykinin neuropeptides is not only providing an understanding of the mechanism of amyloid fibril formation in general, but also offering plausible explanation that why these neuropeptide might reduce the cytotoxicity associated with Alzheimer's disease related amyloids.
 
Publisher HUMANA PRESS INC
 
Date 2014-10-15T08:03:53Z
2014-10-15T08:03:53Z
2012
 
Type Article
 
Identifier CELL BIOCHEMISTRY AND BIOPHYSICS, 64(1)29-44
http://dx.doi.org/10.1007/s12013-012-9364-z
http://dspace.library.iitb.ac.in/jspui/handle/100/14635
 
Language en