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Correlation between pellet morphology and glycopeptide antibiotic balhimycin production by Amycolatopsis balhimycina DSM 5908

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Title Correlation between pellet morphology and glycopeptide antibiotic balhimycin production by Amycolatopsis balhimycina DSM 5908
 
Creator SINGH, KP
WANGIKAR, PP
JADHAV, S
 
Subject Antibiotic production
Mycelia
Submerged cultivation
Substrate consumption
HYGROSCOPICUS VAR.-GELDANUS
CITRIC-ACID PRODUCTION
IMAGE-ANALYSIS
MYCELIAL MORPHOLOGY
FILAMENTOUS MICROORGANISMS
SUBMERGED CULTIVATIONS
METABOLITE PRODUCTION
BATCH FERMENTATION
CULTURE
GROWTH
 
Description Actinomycetes, a class of filamentous bacteria, are an important source of several industrially relevant secondary metabolites. Several environmental factors including the media composition affect both biomass growth and product formation. Likewise, several studies have shown that environmental factors cause changes in cellular morphology. However, the relationship between morphology and product formation is not well understood. In this study, we first characterized the effect of varying concentrations of phosphate and ammonia in defined media on pellet morphology for an actinomycete Amycolatopsis balhimycina DSM 5908, which produces balhimycin, a glycopeptide antibiotic. Our results show that higher balhimycin productivity is correlated with the following morphological features: (1) higher pellet fraction in the biomass, (2) small elongated pellets, and (3) shorter filaments in hyphal growth in the periphery of the pellets. The correlation between morphology and product formation was also observed in industrially relevant complex media. Although balhimycin production starts after 72 h with maximum production around 168 h, the morphological changes in pellets are observed as early as 24 h after commencing of the batch. Therefore, morphology may be used as an early predictor of the end-of-batch productivity. We argue that a similar strategy can be developed for other strains where morphological indicators may be used as a batch monitoring tool.
 
Publisher SPRINGER HEIDELBERG
 
Date 2014-10-15T08:07:26Z
2014-10-15T08:07:26Z
2012
 
Type Article
 
Identifier JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 39(1)27-35
http://dx.doi.org/10.1007/s10295-011-0995-7
http://dspace.library.iitb.ac.in/jspui/handle/100/14642
 
Language en