Record Details

Distribution of live and dead cells in pellets of an actinomycete Amycolatopsis balhimycina and its correlation with balhimycin productivity

DSpace at IIT Bombay

View Archive Info
 
 
Field Value
 
Title Distribution of live and dead cells in pellets of an actinomycete Amycolatopsis balhimycina and its correlation with balhimycin productivity
 
Creator SINGH, KP
MAHENDRA, AL
JAYARAJ, V
WANGIKAR, PP
JADHAV, S
 
Subject Microbial fermentation
Confocal microscopy
Mycelial morphology
Antibiotic production
BATCH FERMENTATION PROCESSES
HYGROSCOPICUS VAR.-GELDANUS
RIFAMYCIN-B FERMENTATION
STREPTOMYCES-ANTIBIOTICUS
MYCELIAL MORPHOLOGY
SURFACE CULTURES
SUBMERGED CULTURES
COELICOLOR
BIOSYNTHESIS
DIFFERENTIATION
 
Description Secondary metabolites such as antibiotics are typically produced by actinomycetes as a response to growth limiting stress conditions. Several studies have shown that secondary metabolite production is correlated with changes observed in actinomycete pellet morphology. Therefore, we investigated the correlation between the production of balhimycin and the spatio-temporal distribution of live and dead cells in pellets of Amycolatopsis balhimycina in submerged cultures. To this end, we used laser scanning confocal microscopy to analyze pellets from balhimycin producing and nonproducing media containing 0.2 and 1.0 g l(-1) of potassium di-hydrogen phosphate, respectively. We observed a substantially higher fraction of live cells in pellets from cultures yielding larger amounts of balhimycin. Moreover, in media that resulted in no balhimycin production, the pellets exhibit an initial death phase which commences from the centre of the pellet and extends in the radial direction. A second growth phase was observed in these pellets, where live mycelia are seen to appear in the dead core of the pellets. This secondary growth was absent in pellets from media producing higher amounts of balhimycin. These results suggest that distribution of live and dead cells and its correlation with antibiotic production in the non-sporulating A. balhimycina differs markedly than that observed in Streptomycetes.
 
Publisher SPRINGER HEIDELBERG
 
Date 2014-10-15T08:07:56Z
2014-10-15T08:07:56Z
2013
 
Type Article
 
Identifier JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 40(2)235-244
http://dx.doi.org/10.1007/s10295-012-1215-9
http://dspace.library.iitb.ac.in/jspui/handle/100/14643
 
Language en